Global transcriptomic analysis of the response of Corynebacterium glutamicum to ferulic acid
Global transcriptomic analysis of the response of Corynebacterium glutamicum to ferulic acid
复制标题
谷氨酸棒杆菌对阿魏酸反应的整体转录组分析
DOI：
10.1007/s00203-016-1306-5
复制
发表时间：
2017-03-01
影响因子：
2.8
通讯作者：
Wang, Yao
中科院分区：
文献类型：
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作者：
Chen, Can;Pan, Junfeng;Wang, Yao
谷氨酸棒杆菌可以以阿魏酸为唯一碳源生存。在这项研究中，我们评估了C。通过全局转录响应分析，研究了谷氨酸对阿魏酸胁迫的反应。转录数据表明，参与阿魏酸降解的几个基因受到影响。此外，与应激反应，蛋白质保护或降解和DNA修复，复制，转录和翻译，以及细胞包膜相关的几个基因的差异表达。C. katA或sigE基因缺失。谷氨酸导致阿魏酸胁迫下细胞活力降低。这些见解将促进进一步工程化的模型工业菌株，具有增强的耐受性阿魏酸，使容易从木质纤维素生产生物燃料。
Corynebacterium glutamicum can survive by using ferulic acid as the sole carbon source. In this study, we assessed the response of C. glutamicum to ferulic acid stress by means of a global transcriptional response analysis. The transcriptional data showed that several genes involved in degradation of ferulic acid were affected. Moreover, several genes related to the stress response; protein protection or degradation and DNA repair; replication, transcription and translation; and the cell envelope were differentially expressed. Deletion of the katA or sigE gene in C. glutamicum resulted in a decrease in cell viability under ferulic acid stress. These insights will facilitate further engineering of model industrial strains, with enhanced tolerance to ferulic acid to enable easy production of biofuels from lignocellulose.