Transgenic tobacco plants that overexpress alfalfa NADH-glutamate synthase have higher carbon and nitrogen content
Transgenic tobacco plants that overexpress alfalfa NADH-glutamate synthase have higher carbon and nitrogen content
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DOI：
10.1093/jexbot/52.364.2079
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发表时间：
2001-11-01
影响因子：
6.9
通讯作者：
Hernández, G
中科院分区：
文献类型：
--
作者：
Chichkova, S;Arellano, J;Hernández, G
本工作报道了组成性过表达NADH-GOGAT的转基因烟草（Nicotiana tabacum L.）植株的特性。研究了3个独立的转化子GOS10、GOS13和GOS19 （bar - GAT下的GO）与CaMV 35S启动子的稳定整合。该转基因被稳定地整合并被后代遗传。在这些GOS系中，NADH-GOGAT mRNA和蛋白在根和叶中表达水平较低，而寄主烟草NADH-GOGAT基因的表达几乎未检测到。与对照植株相比，GOS系根系的酶活性提高了15-40%。在温室条件下，以硝态氮或铵态氮作为唯一氮源，与对照植株相比，在植株进入花期时，茎部总碳和总氮含量较高，茎部干重增加。观察到的GOS植物表型被解释为由于较高的NADH-GOGAT活性而反映出更高的氮同化能力。
This work reports the characterization of transgenic tobacco (Nicotiana tabacum L.) plants that constitutively overexpress NADH-GOGAT. Three independent transformants, designated GOS10, GOS13 and GOS19 (for (GO) under bar GAT (s) under bar ense), with stable integration of the chimeric alfalfa NADH-GOGAT gene fused to the CaMV 35S promoter were studied. The transgene was stably integrated and inherited by the progeny. In these GOS lines, the expression of NADH-GOGAT mRNA and protein was detected at low levels in roots and leaves, while the expression of the host tobacco NADH-GOGAT gene was nearly undetectable. The roots of GOS lines showed an elevated (15-40%) enzyme activity as compared to control plants. When GOS plants were grown under greenhouse conditions and fed with either nitrate or ammonium as the sole nitrogen source, they showed higher total carbon and nitrogen content in shoots and increased shoot dry weight when plants were entering into the flowering stage, as compared to control plants. The observed phenotype of GOS plants was interpreted as reflecting a higher capacity to assimilate nitrogen due to a higher NADH-GOGAT activity.