New insights into the role of spermine in Arabidopsis thaliana under long-term salt stress
New insights into the role of spermine in Arabidopsis thaliana under long-term salt stress
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DOI：
10.1016/j.plantsci.2011.03.013
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发表时间：
2012-01-01
期刊：
影响因子：
5.2
通讯作者：
Ruiz, Oscar A.
中科院分区：
文献类型：
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作者：
Alet, Analia I.;Sanchez, Diego H.;Ruiz, Oscar A.
多胺（腐胺、亚精胺和精胺）通常与植物对环境信号的反应有关。游离精胺积累被认为是长期盐胁迫的一个特殊特征，在模式植物拟南芥中，精胺合成酶基因（AtSPMS）已被报道可被脱落酸（ABA）和急性盐胁迫处理诱导。为了揭示游离精胺在盐胁迫下的生理作用，我们分析了拟南芥盐超敏sos突变体（盐覆盖敏感突变体sos1-1、sos1-1和sos1-1）的多胺代谢，并研究了这些突变体在精胺和热精胺合成方面的盐胁迫耐受性（acl5-1、spms-1和acl5-1/spms-1）。本文的研究结果表明，多胺代谢的诱导是一种与sos无关的盐度反应，并且在敏感背景下被全局过度诱导。此外，在长期盐度下，精胺和热精胺合成突变体（acl5-1、spms-1和双acl5-1/spms-1）积累了更多的Na+，在生存实验中表现比WT差。因此，支持这些高级多胺在耐盐机制中的作用。2011爱思唯尔爱尔兰有限公司版权所有。
Polyamines (putrescine, spermidine and spermine) are traditionally implicated in the response of plants to environmental cues. Free spermine accumulation has been suggested as a particular feature of long-term salt stress, and in the model plant Arabidopsis thaliana the spermine synthase gene (AtSPMS) has been reported as inducible by abscisic acid (ABA) and acute salt stress treatments. With the aim to unravel the physiological role of free spermine during salinity, we analyzed polyamine metabolism in A. thaliana salt-hypersensitive sos mutants (salt overlay sensitive; sos1-1, sos2-1 and sos3-1), and studied the salt stress tolerance of the mutants in spermine and thermospermine synthesis (acl5-1, spms-1 and acl5-1/spms-1). Results presented here indicate that induction in polyamine metabolism is a SOS-independent response to salinity and is globally over-induced in a sensitive background. In addition, under long-term salinity, the mutants in the synthesis of spermine and thermospermine (acl5-1, spms-1 and double acl5-1/spms-1) accumulated more Na+ and performed worst than WT in survival experiments. Therefore, support is given to a role for these higher polyamines in salt tolerance mechanisms. (C) 2011 Elsevier Ireland Ltd. All rights reserved.