Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague-Dawley rats
Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague-Dawley rats
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DOI：
10.1080/08958370701432108
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发表时间：
2007-01-01
影响因子：
2.1
通讯作者：
Yu, Il Je
中科院分区：
文献类型：
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作者：
Ji, Jun Ho;Jung, Jae Hee;Yu, Il Je
银纳米粒子的抗菌作用使其在医疗保健、电子和家居产品中得到了广泛的应用。因此，随着应用程序的扩展，暴露人群继续增加。虽然以前的研究银粉尘，烟雾，和银化合物已经揭示了一些见解，还知之甚少的纳米尺寸的银颗粒的毒性，其中的大小和表面积被认为是重要的决定因素的毒性。因此，银纳米颗粒的吸入毒性是确保工作人员和消费者健康所特别关注的问题。然而，可吸入环境纳米尺寸颗粒的分散已经成为评估纳米尺寸颗粒的吸入对呼吸系统的影响的障碍。因此，本研究使用了一种装置，该装置使用小型陶瓷加热器通过蒸发/冷凝产生银纳米颗粒。因此，发生器能够将所需浓度的银纳米颗粒分配到含有实验动物的室中。纳米粒子的浓度和分布相对于大小也直接使用微分迁移率分析仪和超细凝聚粒子计数器测量。因此，在28天的时间内测试了银纳米颗粒的吸入毒性。8周龄大鼠，雄性体重约283 g，雌性体重约192 g，分为4组（每组10只大鼠）：新鲜空气对照组，低剂量组（1.73 × 10（4）/cm（3））、中剂量组（1.27 × 10（5）/cm（3））和高剂量组（1.32 × 10（6）颗粒/cm（3），61 μ g/m3）。将动物暴露于银纳米颗粒6小时/天，5天/周，总共4周。在28天的实验中，雄性和雌性大鼠的体重相对于银纳米颗粒的浓度没有显示出任何显著变化。此外，雄性或雌性大鼠的血液学和血液生化值均无显著变化。因此，初步结果表明，暴露于浓度接近当前美国政府工业卫生学家会议（ACGIH）银粉尘限值（100 μ g/m3）的银纳米颗粒似乎没有任何显著的健康影响。
The antibacterial effect of silver nanoparticles has resulted in their extensive application in health, electronic, and home products. Thus, the exposed population continues to increase as the applications expand. Although previous studies on silver dust, fumes, and silver compounds have revealed some insights, little is yet known about the toxicity of nano-sized silver particles, where the size and surface area are recognized as important determinants for toxicity. Thus, the inhalation toxicity of silver nanoparticles is of particular concern to ensure the health ofworkers and consumers. However, the dispersion of inhalable ambient nano-sized particles has been an obstacle in evaluating the effect of the inhalation of nano-sized particles on the respiratory system. Accordingly, the present study used a device that generates silver nanoparticles by evaporation/ condensation using a small ceramic heater. As such, the generator was able to distribute the desired concentrations of silver nanoparticles to chambers containing experimental animals. The concentrations and distribution of the nanoparticles with respect to size were also measured directly using a differential mobility analyzer and ultrafine condensation particle counter. Therefore, the inhalation toxicity of silver nanoparticles was tested over a period of 28 days. Eight-week-old rats, weighing about 283 g for the males and 192 g for the females, were divided into 4 groups (10 rats in each group): a fresh-air control, a low-dose group (1.73 x 10(4)/ cm(3)), a middle- dose group (1.27 x 10(5)/ cm(3)), and a high-dose group (1.32 x 10(6) particles/ cm(3), 61 mu g/ m(3)). The animals were exposed to the silver nanoparticles for 6 h/ day, 5 days/ wk, for a total of 4 wk. The male and female rats did not showany significant changes in body weight relative to the concentration of silver nanoparticles during the 28-day experiment. Plus, there were no significant changes in the hematology and blood biochemical values in either the male or female rats. Therefore, the initial results indicated that exposure to silver nanoparticles at a concentration near the current American Conference of Governmental Industrial Hygienists (ACGIH) silver dust limit (100 mu g/ m3) did not appear to have any significant health effects.