Cobalt-bleomycins and deoxyribonucleic acid: sequence-dependent interactions, action spectrum for nicking, and indifference to oxygen.
Cobalt-bleomycins and deoxyribonucleic acid: sequence-dependent interactions, action spectrum for nicking, and indifference to oxygen.
复制标题
钴博莱霉素和脱氧核糖核酸：序列依赖性相互作用、切口作用谱以及对氧的冷漠。
DOI：
10.1021/bi00305a028
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Meares,CF
中科院分区：
文献类型：
--
作者：
Chang,CH;Meares,CF
摘要：在某些碱基序列下，共博莱菌素光诱导DNA链断裂的可能性比其他碱基序列更大。用32P末端标记的DNA限制性片段作为切割底物，在高分辨聚丙烯酰胺凝胶上对产物进行分析，并与铁-博莱霉素产生的产物进行了比较。结果表明，在两种情况下，DNA的损伤部位是相似的：位于鸟嘌呤3‘端的嘧啶残基优先受到攻击。与观察到的划痕特异性一致，在硫酸二甲酯甲基化实验中，发现了三核苷酸序列GGT中钴-博莱霉素与鸟嘌呤残基之间的相互作用。博莱霉素(BLM)1**是临床上用于治疗癌症的一组糖肽抗生素的名称(Umezawa等人，1966；Blum等人，1973；Crooke&Bradner，1976)；该药物被认为是通过化学降解细胞DNA在体内发挥作用(Suzuki等人，1969；Terasima等人，1970)。
C.-H. Chang and CF Meares* abstract: Light-induced strand scission of DNA by co-balt-bleomycins is more likely to occur at certain base se-quences than others. By use of 32P-end-labeled DNA re-striction fragments as the substrates for cleavage, products have been analyzed on high-resolution polyacrylamide gels and compared to those produced by iron-bleomycin. The results indicate that the sites of damage to DNA are similar in both cases: pyrimidine residues located at the 3'side of a guanine are preferentially attacked. Consistent with the observed nicking specificity, interactions between cobalt-bleomycin and guanine residues in the trinucleotide sequence GGT are re-vealed in a dimethyl sulfate methylation experiment. TheBleomycin (BLM) 1** is the name of a group of glycopeptide antibiotics used clinically in the treatment of cancer (Umezawa et al., 1966; Blum et al., 1973; Crooke & Bradner, 1976); the drug is thought to act in vivo by chemically degrading cellular DNA (Suzuki et al., 1969; Terasima et al., 1970).