Enhanced M1/M2 Macrophage Ratio Promotes Orthodontic Root Resorption
Enhanced M1/M2 Macrophage Ratio Promotes Orthodontic Root Resorption
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增强的 m1/m2 巨噬细胞比率可促进正畸牙根吸收。
DOI：
10.1177/0022034514553817
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发表时间：
2015-01-01
影响因子：
7.6
通讯作者：
Zhou, Y.
中科院分区：
文献类型：
--
作者：
He, D.;Kou, X.;Zhou, Y.
机械力引起的正畸牙根吸收是正畸治疗中的一个主要临床挑战。巨噬细胞在正畸牙根吸收中起重要作用，但其机制尚不清楚。在这项研究中，我们研究的机制，其中M1到M2巨噬细胞极化的比例影响牙根吸收正畸牙齿移动。镍钛螺旋弹簧作用于大鼠上颌第一磨牙3 ~ 14 d后，牙根发生吸收。阳性染色破牙细胞或破骨细胞与抗酒石酸酸性磷酸酶被发现在吸收区。同时，M1样巨噬细胞CD 68和诱导型一氧化氮合酶（iNOS）阳性的持续积累在牙周组织的压缩侧。此外，M1激活因子干扰素-γ和M1相关促炎细胞因子肿瘤坏死因子（TNF）-α的表达在牙周组织的压缩侧上调。当施加正畸力后第14天去除螺旋弹簧时，牙根吸收部分被挽救。CD 68（+）CD 163（+）M2样巨噬细胞在牙周组织受压侧逐渐增多。M2激活剂白细胞介素（IL）-4和M2相关抗炎细胞因子IL-10的水平也增加。全身注射TNF-α抑制剂依那西普或IL-4可减轻牙根吸收的严重程度，并降低M1与M2巨噬细胞的比例。这些数据暗示M1和M2巨噬细胞之间的平衡影响正畸牙根吸收。根吸收加剧了增强M1/M2的比例，但部分救出了减少M1/M2的比例。
Mechanical force-induced orthodontic root resorption is a major clinical challenge in orthodontic treatment. Macrophages play an important role in orthodontic root resorption, but the underlying mechanism remains unclear. In this study, we examined the mechanism by which the ratio of M1 to M2 macrophage polarization affects root resorption during orthodontic tooth movement. Root resorption occurred when nickel-titanium coil springs were applied on the upper first molars of rats for 3 to 14 d. Positively stained odontoclasts or osteoclasts with tartrate-resistant acid phosphatase were found in resorption areas. Meanwhile, M1-like macrophages positive for CD68 and inducible nitric oxide synthase (iNOS) persistently accumulated on the compression side of periodontal tissues. In addition, the expressions of the M1 activator interferon-gamma and the M1-associated pro-inflammatory cytokine tumor necrosis factor (TNF)-alpha were upregulated on the compression side of periodontal tissues. When the coil springs were removed at the 14th day after orthodontic force application, root resorption was partially rescued. The number of CD68(+)CD163(+) M2-like macrophages gradually increased on the compression side of periodontal tissues. The levels of M2 activator interleukin (IL)-4 and the M2-associated anti-inflammatory cytokine IL-10 also increased. Systemic injection of the TNF-alpha inhibitor etanercept or IL-4 attenuated the severity of root resorption and decreased the ratio of M1 to M2 macrophages. These data imply that the balance between M1 and M2 macrophages affects orthodontic root resorption. Root resorption was aggravated by an enhanced M1/M2 ratio but was partially rescued by a reduced M1/M2 ratio.