Use of Bodipy-labeled sphingolipid and cholesterol analogs to examine membrane microdomains in cells.
Use of Bodipy-labeled sphingolipid and cholesterol analogs to examine membrane microdomains in cells.
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DOI：
10.1007/s00418-008-0509-5
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发表时间：
2008-11
影响因子：
2.3
通讯作者：
Pagano, Richard E.
中科院分区：
文献类型：
--
作者：
Marks, David L.;Bittman, Robert;Pagano, Richard E.
大量证据表明细胞膜，如质膜，包含多个不同的脂质和蛋白质组成和功能的“微域”。这些结构域有时富含胆固醇和鞘脂，被认为是调节许多生物和病理过程的重要结构。本文综述了利用荧光（Bodipy）标记的鞘脂和胆固醇类似物来研究这些结构域。我们讨论了bodipy -胆固醇和天然胆固醇在人工双分子层和培养细胞中的行为之间的相似性，以及基于bodipy -荧光团的浓度依赖的单体准分子荧光特性，使用bodipy -鞘脂类似物在活细胞中可视化膜结构域。强调了使用bodipy -d- red -乳糖神经酰胺检测质膜和核内体膜上的结构域，并讨论了鞘脂立体化学在调节结构域形成中的重要性。最后，我们认为bodipy -鞘脂可能在未来的研究中有用，以检查细胞表面的膜结构域与质膜内叶上其他脂质和蛋白质富集的结构域之间的关系。
Much evidence has accumulated to show that cellular membranes such as the plasma membrane, contain multiple “microdomains” of differing lipid and protein composition and function. These domains are sometimes enriched in cholesterol and sphingolipids and are believed to be important structures for the regulation of many biological and pathological processes. This review focuses on the use of fluorescent (Bodipy) labeled analogs of sphingolipids and cholesterol to study such domains. We discuss the similarities between the behavior of Bodipy-cholesterol and natural cholesterol in artificial bilayers and in cultured cells, and the use of Bodipy-sphingolipid analogs to visualize membrane domains in living cells based on the concentration-dependent monomer-excimer fluorescence properties of the Bodipy-fluorophore. The use of Bodipy-d-erythro-lactosylceramide is highlighted for detection of domains on the plasma membrane and endosome membranes, and the importance of the sphingolipid stereochemistry in modulating domain formation is discussed. Finally, we suggest that Bodipy-sphingolipids may be useful in future studies to examine the relationship between membrane domains at the cell surface and domains enriched in other lipids and proteins on the inner leaflet of the plasma membrane.
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影响因子：
3.3
作者：
Cheng, Zhi-Jie;Singh, Raman Deep;Pagano, Richard E.
通讯作者：
Pagano, Richard E.
影响因子：
2.9
作者：
FIEDLER, K;KOBAYASHI, T;SIMONS, K
通讯作者：
SIMONS, K
影响因子：
2.9
作者：
CHATTOPADHYAY, A;LONDON, E
通讯作者：
LONDON, E
影响因子：
3.2
作者：
Hakomori, S
通讯作者：
Hakomori, S
影响因子：
3.3
作者：
Choudhury, A;Sharma, DK;Pagano, RE
通讯作者：
Pagano, RE