Adenine nucleotide pool size, adenine nucleotide translocase activity, and respiratory activity in newborn rabbit liver mitochondria.
Adenine nucleotide pool size, adenine nucleotide translocase activity, and respiratory activity in newborn rabbit liver mitochondria.
复制标题
新生兔肝线粒体中的腺嘌呤核苷酸池大小、腺嘌呤核苷酸转位酶活性和呼吸活性。
DOI：
10.1016/0005-2728(82)90035-4
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Aprille,JR
中科院分区：
文献类型：
--
作者：
Rulfs,J;Aprille,JR
新生兔肝线粒体腺嘌呤核苷酸含量(ATP+ADP+AMP)在出生时为6.0±0.5nmol/mg线粒体蛋白，出生后2 h迅速上升至14.5±1.7nmol/mg蛋白，6 h达高峰，4 d后逐渐下降至7.8±0.6 nmol/mg蛋白。线粒体中腺嘌呤核苷酸池的大小与腺嘌呤核苷酸转位酶活性呈显著正相关(r=0.82)。相比之下，谷氨酸+苹果酸支持的状态3呼吸频率从出生到出生第一周保持不变。状态4的速率也保持不变，呼吸控制指数和非耦合呼吸速率也保持不变。结论如下：(1)转位酶的最大活性受线粒体内腺嘌呤核苷酸池大小的限制。(2)在新生兔肝线粒体中，状态3呼吸频率不受腺苷池大小或转位酶介导腺嘌呤交换的最大容量的限制。(3)与大鼠相比，兔肝线粒体在出生时在呼吸频率和氧化磷酸化方面具有完全的功能。(4)新生哺乳动物肝脏线粒体在出生后迅速积累腺嘌呤核苷酸，这可能是新生哺乳动物正常代谢调节的一个普遍特征。
The adenine nucleotide content (ATP+ADP+AMP) of newborn rabbit liver mitochondria was 6.0±0.5 nmol/mg mitochondrial protein at birth, increased rapidly to 14.5±1.7 nmol/mg protein by 2 h postnatal, peaked at 6 h, then decreased gradually to 7.8±0.6 nmol/mg protein by 4 days postnatal. There was a strong positive correlation (r=0.82) between the total adenine nucleotide pool size and adenine nucleotide translocase activity in these mitochondria. In contrast, glutamate + malate-supported State 3 respiratory rates remained constant from birth through the first week of life. State 4 rates also remained constant, as did the respiratory control index and uncoupled respiratory rates. The following conclusions are suggested: (1) The maximum rate of translocase activity is limited by the intramitochondrial adenine nucleotide pool size. (2) In newborn rabbit liver mitochondria, the State 3 respiratory rate is not limited by either the adenine pool size or the maximum capacity for translocase-mediated adenine exchange. (3) In contrast to rat, rabbit liver mitochondria are fully functional at birth with regard to respiratory rates and oxidative phosphorylation. (4) The rapid postnatal accumulation of adenine nucleotides by liver mitochondria, now documented in two species, may be a general characteristic of normal metabolic adjustment in neonatal mammals.