Phylogeography of the wild Lager-brewing ancestor (Saccharomyces eubayanus) in Patagonia
Phylogeography of the wild Lager-brewing ancestor (Saccharomyces eubayanus) in Patagonia
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DOI：
10.1111/1462-2920.14375
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发表时间：
2018-10-01
影响因子：
5.1
通讯作者：
Libkind, Diego
中科院分区：
文献类型：
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作者：
Eizaguirre, Juan I.;Peris, David;Libkind, Diego
Saccharomyces eubayanus 是啤酒酿造酵母的近亲，首先在北巴塔哥尼亚发现，与黄毛榉树有关。近年来，在北美、亚洲和新西兰发现了更多菌株，基因组分析表明存在这种酵母的两个主要种群，它们都存在于巴塔哥尼亚。在这里，我们对巴塔哥尼亚自然环境中的 S. eubayanus 进行了最全面的研究（400 个样本），并证实该地区对该物种的分离成功率是全世界最高的（超过 10 倍）。 200 个分离株（COX2、DCR1、intFR）的遗传特征揭示了五个地理结构亚群。我们假设海洋入侵和冰川作用塑造了巴塔哥尼亚的地貌，从而导致了人口分化。对 60 个野生 S. eubayanus 菌株发酵性能的首次大规模筛选表明哪些亚群更适合啤酒生产。
Saccharomyces eubayanus is the close relative of the Lager-brewing yeast and was firstly found in North Patagonia associated with Nothofagus trees. In recent years additional strains were found in North America, Asia and New Zealand, and genomic analyses showed the existence of two main populations of this yeast, both of them present in Patagonia. Here, we performed the most comprehensive study of S. eubayanus in Patagonia natural environments (400 samples) and confirmed that this region has the highest isolation success rate for this species described worldwide (more than 10-fold). The genetic characterization of 200 isolates (COX2, DCR1, intFR) revealed five geographically structured subpopulations. We hypothesized that marine ingressions and glaciations, which shaped the Patagonian landscape, contributed on population differentiation. The first large screening of fermentation performance of 60 wild S. eubayanus strains indicated which subpopulations would be more suitable for beer production.