Cytokinetic bridge triggers de novo lumen formation in vivo
Cytokinetic bridge triggers de novo lumen formation in vivo
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DOI：
10.1038/s41467-020-15002-8
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发表时间：
2020-03-09
影响因子：
16.6
通讯作者：
Hehnly, H.
中科院分区：
文献类型：
--
作者：
Rathbun, L., I;Colicino, E. G.;Hehnly, H.
多细胞轮状体是在不同器官形成期间充当中间体的瞬时上皮结构。我们已经确定了一个独特的贡献者，在斑马鱼Kupffer囊泡（KV），需要细胞分裂，特别是有丝分裂的最后阶段，称为分裂的玫瑰花结形成。KV利用玫瑰花结作为形成由纤毛上皮细胞包围的管腔之前的先决条件。我们的研究表明，KV-注定的细胞保持相互连接的细胞动力学桥梁，位置在玫瑰花结的中心。这些桥作为一个里程碑，指导Rab 11囊泡运动，以提供一个必要的货物管腔形成，CFTR（囊性纤维化跨膜传导调节）。在这里，我们报告了通过激光消融或使用Rab 11的光遗传学聚类抑制解离导致破坏的管腔形成的过早桥分裂。我们提出了一个模型，其中KV有丝分裂细胞战略性地将其细胞动力学桥放置在玫瑰花结中心，Rab 11相关囊泡转运CFTR以帮助管腔建立。
Multicellular rosettes are transient epithelial structures that serve as intermediates during diverse organ formation. We have identified a unique contributor to rosette formation in zebrafish Kupffer's vesicle (KV) that requires cell division, specifically the final stage of mitosis termed abscission. KV utilizes a rosette as a prerequisite before forming a lumen surrounded by ciliated epithelial cells. Our studies identify that KV-destined cells remain interconnected by cytokinetic bridges that position at the rosette's center. These bridges act as a landmark for directed Rab11 vesicle motility to deliver an essential cargo for lumen formation, CFTR (cystic fibrosis transmembrane conductance regulator). Here we report that premature bridge cleavage through laser ablation or inhibiting abscission using optogenetic clustering of Rab11 result in disrupted lumen formation. We present a model in which KV mitotic cells strategically place their cytokinetic bridges at the rosette center, where Rab11-associated vesicles transport CFTR to aid in lumen establishment.