In situ modification of the phospholipid environment of native rabbit sarcoplasmic reticulum membranes.
In situ modification of the phospholipid environment of native rabbit sarcoplasmic reticulum membranes.
复制标题
原位修饰天然兔肌质网膜的磷脂环境。
DOI：
10.1006/bbrc.1994.1441
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发表时间：
1994
影响因子：
3.1
通讯作者：
Mendelsohn,R
中科院分区：
文献类型：
--
作者：
Szalontai,B;Vigh,L;Joo,F;Senak,L;Mendelsohn,R
含氘环境中的水溶性氢化催化剂（二（茜素单磺酸钠）钯）已用于将氘原子原位插入生物膜的脂肪酰基链中。通过傅里叶变换红外光谱检测所形成的C-D键的伸缩振动的热致响应被用作生物膜结构的选择性探针。不饱和脂肪酰基链的部分氘化与红外检测相结合，可能提供一种检测特定脂质类别的特定生物学作用的方法。在目前对肌浆网膜和纯化磷脂/CaATP酶囊泡的研究中，还表明νC-D监测特定膜位置的变化，这些变化可能通过CH2对称拉伸频率（一种广泛使用的红外光谱参数）未被检测到。后者反映了酰基链的平均环境。这里描述的方法可能适合生物膜研究的广泛应用。
A water soluble hydrogenation catalyst (palladium di(sodium alizarine monosulphonate)) in a deuterium-containing environment has been used for thein situinsertion of deuterium atoms into the fatty acyl chains of biological membranes. The thermotropic response of the stretching vibrations of the formed C-D bonds, as detected by Fourier transform IR spectroscopy, was used as a selective probe of biological membrane structure. Partial deuteration of unsaturated fatty acyl chains coupled with IR detection potentially provides a means for detecting specific biological roles of particular lipid classes. In the current study of sarcoplasmic reticulum membranes and purified phospholipid/CaATPase vesicles, it is also shown that νC-D monitors change at particular membrane locations which may remain undetected through the CH2symmetric stretching frequency, a widely used IR spectral parameter. The latter reflects the average environment of the acyl chains. The approach described here may be suitable for wide applications to the study of biomembranes.