HIV-1 evolution following transmission to an HLA-B*5801-positive patient.
HIV-1 evolution following transmission to an HLA-B*5801-positive patient.
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HIV-1 传播给 HLA-B*5801 阳性患者后的演变。
DOI：
10.1086/648377
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Blankson,JoelN
中科院分区：
文献类型：
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作者：
O'Connell,KarenA;Xu,Jie;Durbin,AnnaP;Apuzzo,LindaG;Imteyaz,Hejab;Williams,ThomasM;Ray,StuartC;Margolick,JosephB;Siliciano,RobertF;Blankson,JoelN
具有HLA-B*57/5801等位基因的患者自发控制病毒复制的人类免疫缺陷病毒1型（HIV-1）特异性免疫应答是基于T细胞的HIV-1疫苗的重要模型。因此，确定这种反应的广度和这些患者原发性感染的病毒逃逸程度至关重要。在这里，我们记录了3个HLA-B*5801限制性表位突变的发展，在感染后第167天，HLA-B*5801阳性患者的病毒载量仅为1159拷贝/mL。进行全基因组序列分析以确定HLA-B*5801限制性表位的突变程度，并将特定基因的纵向序列数据与关键表位的酶联免疫斑点分析相结合以确定逃逸突变的重要性。因此，尽管细胞毒性T淋巴细胞表位内的多个逃逸突变迅速发展，但病毒复制的相对控制可以维持
The human immunodeficiency virus type 1 (HIV-1)–specific immune responses of patients with the HLA-B*57/5801 alleles who spontaneously control viral replication serve as an important model for T cell–based HIV-1 vaccines. Determining the breadth of this response and the extent of virologic escape in primary infection in these patients is therefore critical. Here we document the development of mutations in 3 HLA-B*5801-restricted epitopes ingag,nefandpolin an HLA-B*5801-positive patient who had a viral load of only 1159 copies/mL at day 167 after infection. A full genome sequence analysis was performed to determine the extent of mutations in HLA-B*5801-restricted epitopes, and longitudinal sequence data of specific genes were combined with enzyme-linked immunospot assay analysis of critical epitopes to determine the importance of escape mutations. Thus, relative control of viral replication can be maintained in spite of the rapid development of multiple escape mutations within cytotoxic T lymphocyte epitopes