Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration.
Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration.
复制标题
含有纳米磷酸镁和小麦蛋白的生物复合支架的体外和体内降解性、生物相容性和成骨作用，用于骨再生
DOI：
10.2147/ijn.s105645
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发表时间：
2016
影响因子：
8
通讯作者：
Xu S
中科院分区：
文献类型：
--
作者：
Xia Y;Zhou P;Wang F;Qiu C;Wang P;Zhang Y;Zhao L;Xu S
本研究制备了具有生物活性的纳米磷酸镁(nMP)/小麦蛋白（WP）复合材料（MWC）支架。结果表明，MWC支架不仅具有400 ~ 600 μm的大孔，而且在大孔壁上具有10 ~ 20 μm的微孔。含有40 w% nMP的MWC支架在磷酸盐缓冲盐水中具有适当的可降解性，并产生弱碱性微环境。在细胞培养实验中，结果显示MWC支架可显著促进MC3T3-E1细胞的增殖、分化和向支架内生长。同步辐射微计算机断层扫描和体内植入组织切片分析结果显示，与WP支架相比，MWC支架明显促进了新骨的形成和骨缺损的修复。此外，我们发现随着MWC支架中残留材料的逐渐减少，新形成的骨组织持续增加。此外，免疫组化分析进一步证实了MWC支架对成骨相关细胞分化和新生骨再生的刺激作用。结果表明，MWC支架具有良好的生物相容性和可降解性，可促进体内成骨，具有修复骨组织的潜力。
In this study, bioactive scaffold of nano magnesium phosphate (nMP)/wheat protein (WP) composite (MWC) was fabricated. The results revealed that the MWC scaffolds had interconnected not only macropores (sized 400–600 μm) but also micropores (sized 10–20 μm) on the walls of macropores. The MWC scaffolds containing 40 w% nMP had an appropriate degradability in phosphate-buffered saline and produced a weak alkaline microenvironment. In cell culture experiments, the results revealed that the MWC scaffolds significantly promoted the MC3T3-E1 cell proliferation, differentiation, and growth into the scaffolds. The results of synchrotron radiation microcomputed tomography and analysis of the histological sections of the in vivo implantation revealed that the MWC scaffolds evidently improved the new bone formation and bone defects repair as compared with WP scaffolds. Moreover, it was found that newly formed bone tissue continued to increase with the gradual reduction of materials residual in the MWC scaffolds. Furthermore, the immunohistochemical analysis further offered the evidence of the stimulatory effects of MWC scaffolds on osteogenic-related cell differentiation and new bone regeneration. The results indicated that MWC scaffolds with good biocompability and degradability could promote osteogenesis in vivo, which would have potential for bone tissue repair.