The influence mechanism of DO on the microbial community and carbon source metabolism in two solid carbon source systems
The influence mechanism of DO on the microbial community and carbon source metabolism in two solid carbon source systems
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DO对两种固体碳源系统微生物群落和碳源代谢的影响机制
DOI：
10.1016/j.envres.2021.112410
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发表时间：
2022
影响因子：
8.3
通讯作者：
Chu Zhang
中科院分区：
文献类型：
--
作者：
Heng Wu;Qian Zhang;Xue Chen;Yunan Zhu;Chunbo Yuan;Chu Zhang
固体碳源同步硝化反硝化（SND）工艺参数对微生物和碳源代谢的调控机制尚不清楚。研究了溶解氧（DO）和生物可降解聚合物（BDPs）类型（聚己内酯（PCL）和聚丁二酸丁二醇酯（PBS））对处理效果和微生物特性的影响。结果表明，在DO为5 mg/L的最佳工艺条件下，以PBS和PCL为填料的SND工艺总氮（TN）去除率分别达到93.02%和97.28%。PCL碳源系统中具有脱氮能力的优势菌属为噬氢菌属和噬酸菌属，PBS系统中具有脱氮能力的优势菌属为噬酸菌属和未分类丛枝藻科。PCL中的共代谢网络更复杂，更容易被DO调控。BDPs类型主要影响以硫杆菌和金微菌为节点的共代谢网络，最终导致群落结构的变化。在低DO（1和2 mg/L）条件下，BDP类型对群落结构的影响比DO更显著，而在高DO（5 mg/L）条件下，BDP类型对群落结构的影响不显著。此外，在高溶解氧条件下，硝化作用与碳源降解之间的功能酶分布可能会发生冲突。将DO控制在2 mg-5 mg范围内，可进一步提高碳源利用效率。
The regulation mechanism of parameters on microorganisms and carbon source metabolism of solid carbon source simultaneous nitrification and denitrification (SND) process is not clear. In this paper, the effects of dissolved oxygen (DO) and biodegradable polymer (BDPs) types ((Polycaprolactone, PCL) and (Polybutylene succinate, PBS)) on treatment performance and microbial characteristics were investigated. The results show that the total nitrogen (TN) removal efficiency of SND process using PBS and PCL as fillers reached 93.02% and 97.28% under optimal parameter of DO 5 mg/L, respectively. The dominant genus with nitrogen removal performance in the PCL carbon source system areHydrogenophagaandAcidovorax, and the main genus in the PBS system areAcidovoraxandunclassified_Comamonadaceae. The co-metabolic network in PCL is more complex and easier to be regulated by DO. The BDPs types mainly affect the co-metabolic network with nodes ofThiothrixandChryseomicrobium, ultimately leading to changes in the community structure. By comparison, BDPs types have a more significant impact on community structure than DO under low DO conditions (1 and 2 mg/L), but not under high DO condition(5 mg/L). Further, the distribution of functional enzymes may conflict between nitrification and carbon source degradation under high DO condition. Controlling the DO within the range of 2 mg–5 mg can further improve carbon source utilization efficiency.