Trichinella spiralis infection changes immune response in mice performed abdominal heterotopic cardiac transplantation and prolongs cardiac allograft survival time
Trichinella spiralis infection changes immune response in mice performed abdominal heterotopic cardiac transplantation and prolongs cardiac allograft survival time
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旋毛虫感染改变了进行腹部异位心脏移植的小鼠的免疫反应，并延长了心脏同种异体移植物的存活时间。
DOI：
10.1007/s00436-015-4762-y
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发表时间：
2016-01-01
影响因子：
2
通讯作者：
Ma, Yi
中科院分区：
文献类型：
--
作者：
Deng, Gengguo;Deng, Ronghai;Ma, Yi
同种异体移植排斥反应多年来一直是患者长期存活的障碍。目前治疗移植排斥反应的策略并不像我们预期的那样理想，尤其是对于长期治疗。旋毛虫是一种寄生在哺乳动物肌肉中的线虫，作为一种入侵者，通过逃避宿主的免疫攻击而与宿主长期保持和谐。为探讨旋毛虫感染对同种异体心脏移植排斥反应的影响，以BALB/c(H-2(B))小鼠为供体，C57BL/6(H-2(B))小鼠为受体，建立同种异体心脏移植模型。每日腹部触诊观察移植物存活情况，H&E染色观察移植物组织学变化，流式细胞仪检测移植物中CD4(+)、CD8(+)、CD4(+)干扰素-γ(+)、CD4(+)IL-17(+)T细胞和调节性T细胞。Luminex法检测血清细胞因子水平。旋毛虫组移植心脏平均存活时间为23.40±1.99天，而赋形剂对照组为10.60±0.75天。此外，在实验组小鼠心脏移植模型中，在移植后第7天，我们观察到同种异体心脏移植的组织学改变减轻，相应的CD8(+)T细胞减少，Th1和Th17反应受到抑制，调节性T细胞频率增加。这些数据表明，旋毛虫感染导致小鼠心脏移植后移植物存活时间延长，Th1/Th17反应受到抑制，调节性T细胞增加。
Allograft rejection has been an obstacle for long-term survival of patients for many years. Current strategies for transplant rejection are not as optimal as we expected, especially for long-term treatments. Trichinella spiralis, a nematode parasitized in mammalian muscle and as an invader, maintains harmonious with host in the long term by evading host immune attack. To determine whether T. spiralis infection impacts on allograft rejection, we performed mice cardiac allograft transplantation model by using BALB/c (H-2(b)) mice as donors and C57BL/6 (H-2(b)) mice orally infected with 300 muscle larvae for 28 days as recipients. Graft survival was monitored by daily palpation of the abdomen; histologic change was observed by H&E stain; and CD4(+), CD8(+), CD4(+) IFN-gamma(+), and CD4(+) IL-17(+) T cells and regulatory T cells were examined with the use of flow cytometry. Serum cytokine levels were measured by Luminex. Finally, we found that mean survival time of cardiac allografts in T. spiralis group was 23.40 +/- 1.99 days, while the vehicle control group was 10.60 +/- 0.75 days. Furthermore, we observed alleviated histological changes in the heart allograft, decreased corresponding CD8(+) T cells, suppressed Th1 and Th17 responses, and increased regulatory T cell frequency in a murine cardiac transplantation model at day 7 post-transplantation in experimental group. These data suggest that T. spiralis infection resulted in prolonged allograft survival following murine cardiac transplantation, with suppressed Th1/Th17 responses and augmented regulatory T cells.