Human cytomegalovirus preferentially infects the neoplastic epithelium of colorectal cancer: A quantitative and histological analysis
Human cytomegalovirus preferentially infects the neoplastic epithelium of colorectal cancer: A quantitative and histological analysis
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DOI：
10.1016/j.jcv.2012.04.007
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发表时间：
2012-07-01
影响因子：
8.8
通讯作者：
Chan, Yu-Jiun
中科院分区：
文献类型：
--
作者：
Chen, Hsin-Pai;Jiang, Jeng-Kai;Chan, Yu-Jiun
背景：人巨细胞病毒（HCMV）可能参与人类肿瘤的发生。目的：探讨人巨细胞病毒（HCMV）是否特异性感染结直肠癌（CRC）的肿瘤组织。通过PCR和实时荧光定量PCR检测和定量HCMV DNA。结果：经PCR检测，163例癌旁组织标本中HCMV DNA阳性率为5.6%（14/163），而42.3%（69/163）的癌旁组织标本中HCMV DNA阳性率为5.6%（14/163）（P < 0.0001）。在54个样本对中的定量实时PCR显示肿瘤标本中的病毒拷贝显著高于邻近的非肿瘤组织标本（p < 0.001）。原位杂交结果显示，肿瘤上皮细胞胞浆内可见HCMV核酸。结论：巨细胞病毒优先感染大肠癌的肿瘤上皮细胞。病毒在大肠癌肿瘤上皮微环境中的生存及相互作用机制有待进一步研究。(C)2012年爱思唯尔B。V.保留所有权利。
Background: It has long been suggested that human cytomegalovirus (HCMV) might be involved in human oncogenesis. However, whether HCMV was associated with colorectal cancer (CRC) was still controversial.Objective: To clarify whether HCMV specifically infects the tumorous tissue of CRC.Study design: Paired tumor and adjacent non-neoplastic CRC specimens were collected from 163 patients. HCMV DNA was detected and quantified through PCR and quantitative real-time PCR. Virus location was determined by in situ hybridization (ISH) of formalin-fixed paraffin-embedded tissue sections with an HCMV-specific probe.Results: By PCR, HCMV DNA was detected in 42.3% (69/163) of the tumor specimens, while only 5.6%(14/163) samples of adjacent non-neoplastic tissue were positive for HCMV (p < 0.0001). Quantitative real-time PCR in 54 sample pairs revealed significantly higher viral copies in the tumor specimens than the adjacent non-neoplastic tissue specimens (p < 0.001). By ISH, the nucleic acids of HCMV were detected in the cytoplasm of neoplastic epithelium. No hybridization was detected in the inflammatory infiltrates, submucosa, or other stromal tissues.Conclusions: HCMV preferentially infects the tumor epithelium of CRC. How the virus subsists in and interacts with the microenvironment of tumor epithelium of CRC should be studied. (C) 2012 Elsevier B. V. All rights reserved.