Transforming growth factor-β regulates the expression of anosmin (KAL-1) in human retinal pigment epithelial cells.
Transforming growth factor-β regulates the expression of anosmin (KAL-1) in human retinal pigment epithelial cells.
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DOI：
10.1016/j.cyto.2012.12.019
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发表时间：
2013-03
期刊：
影响因子：
3.8
通讯作者：
Nagineni, Chandrasekharam N.
中科院分区：
文献类型：
--
作者：
Raju, Raghavan;Jian, Bixi;Hooks, John J.;Nagineni, Chandrasekharam N.
In a microarray analysis of human retinal pigment epithelial cells (HRPE) treated with TGF-β, in addition to the alteration of a number of known extracellular matrix (ECM)-related genes regulated by TGF-β, we found a significant increase in the expression of Kallmann Syndrome (KAL)-1 gene, that codes for the protein anosmin-1. Enhanced expression of KAL-1 by TGF-β was validated by real-time PCR analysis. In in vitro experiments, TGF-β receptor inhibitor abolished TGF-β-induced expression of KAL-1. Immunofluorescence staining showed increased presence of anosmin-1 in TGF-β treated HRPE cells, with distinct localization at the intercellular junctions. Treatment of HRPE cells with TGF-β enhanced secretion of anosmin-1 and the release of anosmin-1 was further augmented by heparin sulfate. Enhanced secretion of anosmin-1 in the presence of TGF-β and heparin was also observed in other ocular cells such as corneal epithelial and corneal fibroblast cultures. The role of anosmin-1, a protein with adhesion functions, in retinal structure, function and pathology has not been known and remains to be investigated.
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影响因子：
64.8
作者：
FRANCO, B;GUIOLI, S;BALLABIO, A
通讯作者：
BALLABIO, A
影响因子：
4.3
作者：
Jian, Bixi;Nagineni, Chandrasekharam N.;Raju, Raghavan
通讯作者：
Raju, Raghavan
影响因子：
14.5
作者：
Raju, R;Dalakas, MC
通讯作者：
Dalakas, MC
影响因子：
5.6
作者：
Nagineni, CN;Samuel, W;Hooks, JJ
通讯作者：
Hooks, JJ
DOI：
10.1016/j.bbrc.2006.11.033
发表时间：
2007-01-12
影响因子：
3.1
作者：
Honjo, Yasuyuki;Nagineni, Chandrasekharam N.;Kulkarni, Ashok B.
通讯作者：
Kulkarni, Ashok B.