Injectable and thermosensitive PLGA-g-PEG hydrogels containing hydroxyapatite: preparation, characterization and in vitro release behavior
Injectable and thermosensitive PLGA-g-PEG hydrogels containing hydroxyapatite: preparation, characterization and in vitro release behavior
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DOI：
10.1088/1748-6041/7/2/024107
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发表时间：
2012-04-01
影响因子：
4
通讯作者：
Tarasevich, Barbara J.
中科院分区：
文献类型：
--
作者：
Lin, Genyao;Cosimbescu, Lelia;Tarasevich, Barbara J.
本文报道了一种含有羟基磷灰石（HA）的可注射热敏水凝胶复合材料的设计和表征，该材料由聚乳酸-羟基乙酸-聚乙二醇（PLGA-g-PEG）组成，具有潜在的骨组织工程应用前景。在水凝胶中加入透明质酸可以增强复合材料的机械性能和生物活性。研究了透明质酸对水凝胶性能的影响，包括储存模量、溶胶-凝胶过渡性能、pH和体外染料释放行为。采用扫描电镜（SEM）、x射线衍射（XRD）和傅里叶变换红外光谱（FTIR）对水凝胶复合材料进行了研究。结果表明，在HA的存在下，水凝胶复合材料保持了其溶胶-凝胶过渡性质。水凝胶的储存模量以HA含量依赖的方式增强，并且水凝胶的酸性pH环境被HA中和，这两者都比单独的水凝胶有很大的优势。扫描电镜显示，透明质酸颗粒分散良好，分布在水凝胶基质内。复合材料显示小分子模型染料的缓释长达两周，添加HA后缓释量略有增加。这项工作证明了PLGA-g-PEG和HA的新型热凝胶复合材料的形成，该复合材料可注射并促进控释。
Here we report the design and characterization of injectable and thermosensitive hydrogel composites comprised of poly(lactic acid-co-glycolic acid)-g-poly(ethylene glycol)(PLGA-g-PEG) containing hydroxyapatite (HA) for potential application in bone tissue engineering. Inclusion of HA into the hydrogels would provide both enhanced mechanical properties and bioactivity to the composites. The effects of HA on the properties of the hydrogels were investigated in terms of storage modulus, sol-gel transition properties, pH and in vitro dye release behavior. The hydrogel composites were also studied by scanning electron microscopy (SEM), x-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The results revealed that hydrogel composites preserved their sol-gel transition properties in the presence of HA. The storage modulus of the hydrogels was enhanced in a HA-content dependent manner, and the acidic pH environment of the hydrogel was neutralized by HA, both representing great advantages over the hydrogel alone. SEM images showed that HA particles were well dispersed and distributed within the hydrogel matrix. The composites showed a sustained release of a small molecule model dye for up to two weeks with slight increase of release with addition of HA. This work demonstrates the formation of novel thermogelling composites of PLGA-g-PEG and HA that are injectable and promote controlled release.