Biophysical constraints of optogenetic inhibition at presynaptic terminals.
Biophysical constraints of optogenetic inhibition at presynaptic terminals.
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DOI：
10.1038/nn.4266
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发表时间：
2016-04
影响因子：
25
通讯作者：
Yizhar O
中科院分区：
文献类型：
--
作者：
Mahn M;Prigge M;Ron S;Levy R;Yizhar O
我们研究了使用盐视紫红质、古视紫红质和氯离子传导通道视紫红质在突触前末梢的光遗传学抑制的功效。精确定时激活突触前末梢的古视紫红质和盐视紫红质都减弱了诱发释放。然而，持续的古视紫红质激活与增加的自发释放矛盾。激活氯离子传导通道视紫红质触发神经递质释放后，轻发病。我们的研究结果表明，突触前末梢的生物物理特性决定了光遗传操作的独特边界条件。
We investigated the efficacy of optogenetic inhibition at presynaptic terminals using halorhodopsin, archaerhodopsin and chloride-conducting channelrhodopsins. Precisely timed activation of both archaerhodopsin and halorhodpsin at presynaptic terminals attenuated evoked release. However, sustained archaerhodopsin activation was paradoxically associated with increased spontaneous release. Activation of chloride-conducting channelrhodopsins triggered neurotransmitter release upon light onset. Our results indicate that the biophysical properties of presynaptic terminals dictate unique boundary conditions for optogenetic manipulation.