Differential effect of phosphate starvation on synthetic and division rates of Euglena.
Differential effect of phosphate starvation on synthetic and division rates of Euglena.
复制标题
磷酸盐饥饿对眼虫合成率和分裂率的不同影响。
DOI：
10.1016/s0014-4827(71)80056-3
复制
发表时间：
1971
影响因子：
3.7
通讯作者：
R. Parenti
中科院分区：
文献类型：
--
作者：
F. Parenti;A. Dell'aquila;R. Parenti
磷酸盐饥饿导致眼虫分裂和合成速率的差异性抑制。 RNA 和 DNA 合成速率受到的抑制程度比细胞分裂受到的抑制程度更大。因此，随着细胞分裂，细胞的平均 RNA 和 DNA 含量逐渐降低。蛋白质合成的行为更为复杂：磷酸盐饥饿带来蛋白质合成的完全抑制；然而，这种抑制仅持续10小时，此后合成恢复到相当大的速率。将磷酸盐饥饿的细胞传代到含有适量磷酸盐的培养基中，刺激RNA、DNA和蛋白质的合成，从而使细胞中恢复高水平的这些大分子成分。大分子含量保持较高水平，直到磷酸盐变得有限，然后如上所述下降。因此，通过使用适当磷酸盐浓度的培养基和适当的传代培养方案，可以促使眼虫经历其大分子成分的还原和膨胀循环。讨论了这种循环可能的进化和生态意义。
Phosphate starvation causes a differential inhibition of the division and synthetic rate inEuglena. The rates of RNA and DNA synthesis are inhibited to a greater extent than is cell division. As a consequence, the mean RNA and DNA content of the cells become progressively lower as cells divide. The behaviour of protein synthesis is more complex: Phosphate starvation brings about a complete inhibition of protein synthesis; however, such an inhibition lasts only for 10 h whereafter the synthesis is restored to a considerable rate. Subculturing phosphatestarved cells into a medium containing a proper amount of phosphate stimulates the synthesis of RNA, DNA and protein so that high levels of these macromolecular components are restored in the cells. The macromolecular content remains high until phosphate becomes limiting, then it falls as described above. So, by using a medium of proper phosphate concentration and a proper subculturing schedule,Euglenacan be impelled through a cycle of reduction-and-expansion of its macromolecular components. The possible evolutionary and ecological significance of such a cycle is discussed.