Direct electron transfer of glucose oxidase and biosensing for glucose based on PDDA-capped gold nanoparticle modified graphene/multi-walled carbon nanotubes electrode.
Direct electron transfer of glucose oxidase and biosensing for glucose based on PDDA-capped gold nanoparticle modified graphene/multi-walled carbon nanotubes electrode.
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DOI：
10.1016/j.bios.2013.08.043
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发表时间：
2014-02
影响因子：
12.6
通讯作者：
Yanyan Yu;Zuanguang Chen;Sijing He;Beibei Zhang;Xinchun Li;Meicun Yao
中科院分区：
文献类型：
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作者：
Yanyan Yu;Zuanguang Chen;Sijing He;Beibei Zhang;Xinchun Li;Meicun Yao
在这项工作中，制备了聚二烯基二甲基氯化铵（PDDA）包盖金纳米粒子（AuNPs）功能化石墨烯(G)/多壁碳纳米管（MWCNTs）纳米复合材料。在静电吸引作用下，G/MWCNTs杂化材料可以均匀密集地装饰aunp。新的分层纳米结构可以提供更大的表面积和更有利的电子转移微环境。采用AuNPs/G/MWCNTs纳米复合材料作为葡萄糖氧化酶（GOD）的新型固定化平台。电极与GOD之间实现了直接电子转移（DET）。采用场发射扫描电镜（FESEM）、紫外可见光谱（UV-vis）和循环伏安法（CV）对电化学生物传感器进行了表征。经AuNPs/G/MWCNTs修饰的GOD电极制备的葡萄糖生物传感器具有高灵敏度（29.72 mA M−1cm−2）和低检出限（4.8µM），具有令人满意的分析性能。计算出GOD的非均相电子转移速率常数（ΚS）和表观Michaelis-Menten常数（Km）分别为11.18 s−1和2.09 mM。我们所提出的纳米结构具有令人满意的选择性、可重复性和稳定性，为电极制造和葡萄糖生物传感提供了另一种选择。
In this work, poly (diallyldimethylammonium chloride) (PDDA)-capped gold nanoparticles (AuNPs) functionalized graphene (G)/multi-walled carbon nanotubes (MWCNTs) nanocomposites were fabricated. Based on the electrostatic attraction, the G/MWCNTs hybrid material can be decorated with AuNPs uniformly and densely. The new hierarchical nanostructure can provide a larger surface area and a more favorable microenvironment for electron transfer. The AuNPs/G/MWCNTs nanocomposite was used as a novel immobilization platform for glucose oxidase (GOD). Direct electron transfer (DET) was achieved between GOD and the electrode. Field emission scanning electron microscopy (FESEM), UV–vis spectroscopy and cyclic voltammetry (CV) were used to characterize the electrochemical biosensor. The glucose biosensor fabricated based on GOD electrode modified with AuNPs/G/MWCNTs demonstrated satisfactory analytical performance with high sensitivity (29.72 mA M−1cm−2) and low limit of detection (4.8 µM). The heterogeneous electron transfer rate constant (ΚS) and the apparent Michaelis–Menten constant (Km) of GOD were calculated to be 11.18 s−1and 2.09 mM, respectively. With satisfactory selectivity, reproducibility, and stability, the nanostructure we proposed offered an alternative for electrode fabricating and glucose biosensing.