Differentially expressed messenger RNA isoforms of the human estrogen receptor-alpha gene are generated by alternative splicing and promoter usage.
Differentially expressed messenger RNA isoforms of the human estrogen receptor-alpha gene are generated by alternative splicing and promoter usage.
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DOI：
10.1210/mend.12.12.0209
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发表时间：
1998-12
影响因子：
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通讯作者：
G. Flouriot;C. Griffin;M. Kenealy;V. Sonntag-Buck;F. Gannon
中科院分区：
文献类型：
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作者：
G. Flouriot;C. Griffin;M. Kenealy;V. Sonntag-Buck;F. Gannon
描述了几种新的人雌激素受体-α（hER α）mRNA的分离和表征。与之前鉴定的那些一起，它们总共产生了六种hER alpha mRNA亚型（A-F hER alpha mRNA）。通过多个启动子使用从单个hER α基因产生，所有这些转录物编码共同的蛋白质，但由于5个上游外显子（1B-1F）的选择性剪接，它们的5 '非翻译区不同。这些不同的hER α mRNA亚型的RT-PCR和S1核酸酶图谱分析揭示了hER α基因在人类组织和细胞类型中的表达差异模式。A hER α mRNA是在乳腺或来源于该组织的肿瘤细胞系中检测到的主要同种型。在子宫内膜中，主要形式是A和C hER α mRNA亚型，而C和F hER α mRNA亚型是在卵巢中检测到的主要形式。最后，高水平的E hER α mRNA亚型仅限于肝脏，在女性中表达增加。两者合计，我们的研究结果表明，hER α基因是一个复杂的基因组单位，表现出选择性剪接和启动子的使用，在组织特异性的方式。
The isolation and characterization of several new human estrogen receptor-alpha (hERalpha) mRNAs are described. Together with those previously identified, they give rise to a total of six hERalpha mRNA isoforms (A-F hERalpha mRNAs). Produced from a single hERalpha gene by multiple promoter usage, all these transcripts encode a common protein but differ in their 5'-untranslated region as a consequence of alternative splicing of five upstream exons (1B-1F). RT-PCR and S1 nuclease mapping analysis of these different hERalpha mRNA isoforms revealed a differential pattern of expression of the hERalpha gene in human tissues and cell types. The A hERalpha mRNA is the main isoform detected in mammary glands or in the tumor cell lines derived from this tissue. In endometrium, the predominant forms are the A and C hERalpha mRNA isoforms, whereas the C and F hERalpha mRNA isoforms are the major forms detected in ovary. Finally, high levels of the E hERalpha mRNA isoform are restricted to the liver with an increased expression in females. Taken together, our results demonstrate that the hERalpha gene is a complex genomic unit exhibiting alternative splicing and promoter usage in a tissue-specific manner.