D-aTNA circuit orthogonal to DNA can be operated by RNA input via SNA
D-aTNA circuit orthogonal to DNA can be operated by RNA input via SNA
复制标题
与 DNA 正交的 D-aTNA 电路可以通过 SNA 输入 RNA 来操作
DOI：
10.1002/slct.201701126
复制
发表时间：
2017
期刊：
影响因子：
2.1
通讯作者：
Asanuma Hiroyuki
中科院分区：
文献类型：
--
作者：
Murayama Keiji;Nagao Ryuya;Asanuma Hiroyuki
对于与 DNA 正交的信号放大系统，我们设计了一个仅由 D-aTNA 组成的简化跷跷板门。这个新系统就像 DNA 电路一样，通过立足点交换反应进行信号放大。此外，D-aTNA 回路不受携带与 D-aTNA 互补序列的天然核酸的影响。然而，在存在 SNA 接口的情况下，RNA 信号被转换为 D-aTNA 信号，从而成功激活 D-aTNA 电路。该系统可用于设计不受 DNA 和 RNA 污染影响的信号放大电路。
For a signal amplification system that is orthogonal to DNA, we designed a simplified seesaw gate composed of only D‐aTNA. This new system performed signal amplification by toehold exchange reaction just as the DNA circuit did. Moreover, the D‐aTNA circuit was not affected by natural nucleic acids carrying sequences complementary to the D‐aTNA. In the presence of an SNA interface, however, an RNA signal was converted to D‐aTNA signal, resulting in successful activation of D‐aTNA circuit. This system can be used to design signal‐amplification circuits that are not influenced by contaminating DNA and RNA.