Effects of earthworms on the fate of tetracycline and fluoroquinolone resistance genes of sewage sludge during vermicomposting
Effects of earthworms on the fate of tetracycline and fluoroquinolone resistance genes of sewage sludge during vermicomposting
复制标题
蚯蚓堆肥过程中污泥对四环素和氟喹诺酮抗性基因去向的影响
DOI：
10.1016/j.biortech.2018.03.021
复制
发表时间：
2018-07-01
影响因子：
11.4
通讯作者：
Wu, Nan
中科院分区：
文献类型：
--
作者：
Huang, Kui;Xia, Hui;Wu, Nan
使用传统的污泥处理工艺很难消除污水污泥中存在的多种抗生素抗性基因（ARG）。迄今为止，人们对污泥蚯蚓堆肥过程中 ARG 的命运知之甚少。本研究旨在通过对比有和没有蚯蚓的两种污泥稳定系统，研究蚯蚓对污水污泥蚯蚓堆肥过程中四环素和氟喹诺酮抗性基因以及整合子命运的影响。与没有蚯蚓的对照相比，蚯蚓堆肥显着降低了四环素和氟喹诺酮抗性基因和 int1 的丰度（p < 0.05），并且完全去除了 parC。 ARG 的变化与环境因素、水平基因转移、细菌群落组成和蚯蚓堆肥过程中的蚯蚓有关。此外，蚯蚓强烈影响了编码ARGs和Int1的可能宿主细菌，从而减少了蚯蚓堆肥产品中的致病菌。这些结果表明，蚯蚓堆肥可以有效减少污泥中的四环素和氟喹诺酮类抗性基因。
Diverse antibiotic resistance genes (ARGs) present in sewage sludge are difficult to be eliminated using conventional sludge treatment processes. To date, little remains known on the fate of the ARGs during vermicomposting of sludge. This study aimed to investigate the effect of earthworms on the fate of tetracycline and fluoroquinolone resistance genes, and integrons during vermicomposting of sewage sludge through contrasting two systems of sludge stabilization with and without earthworms. Compared to the control without earthworms, vermicomposting significantly (p < 0.05) decreased the abundances of tetracycline and fluoroquinolone resistance genes and int1, with complete removal for parC. Variations in ARGs were associated with environmental factors, horizontal gene transfer, bacterial community composition, and earthworms during vermicomposting. In addition, earthworms strongly affected the possible host bacteria encoding ARGs and Int1, abating the pathogenic bacteria in vermicomposting product. These results imply that vermicomposting could effectively reduce tetracycline and fluoroquinolone resistance genes in the sludge.