Rapid single-step procedure for isolating total glutenin proteins of wheat flour
Rapid single-step procedure for isolating total glutenin proteins of wheat flour
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DOI：
10.1006/jcrs.1998.0225
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发表时间：
1999-03-01
影响因子：
3.8
通讯作者：
Kovacs, MIP
中科院分区：
文献类型：
--
作者：
Fu, BX;Kovacs, MIP
介绍了一种快速、简便、无单体蛋白污染的面粉中总谷蛋白的分离方法。用来自3个加拿大小麦品种(Glenlea、Neepawa和AC Karma)的面粉对这种一步一溶剂提取技术进行了测试。消除单体蛋白交叉污染的溶剂为0.30小写˜M碘化钠-7.5%正丙醇。这种方法应该有助于对小麦样品进行有效的蛋白质质量筛选。
A rapid and simple procedure is described for isolating total glutenin in flour without monomeric protein contamination. This single-step one solvent extraction technique was tested using flours milled from 3 Canadian wheat cultivars (Glenlea, Neepawa and AC Karma). The solvent which eliminates cross-contamination by monomeric proteins is 0.3 smallcap˜M sodium iodide-7.5% 1-propanol. This method should facilitate the efficient screening of wheat samples for protein quality.