Evolution of extracellular Dpp modulators in insects: The roles of tolloid and twisted-gastrulation in dorsoventral patterning of the Tribolium embryo
Evolution of extracellular Dpp modulators in insects: The roles of tolloid and twisted-gastrulation in dorsoventral patterning of the Tribolium embryo
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DOI：
10.1016/j.ydbio.2010.05.019
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发表时间：
2010-09-01
影响因子：
2.7
通讯作者：
Roth, Siegfried
中科院分区：
文献类型：
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作者：
da Fonseca, Rodrigo Nunes;van der Zee, Maurijn;Roth, Siegfried
在苍蝇和脊椎动物中形成DV轴的BMP梯度的形成需要几种细胞外调节剂，如抑制性蛋白Sog/Chordin、切割Sog/Chordin的金属蛋白酶Tolloid（Tld）和CR结构域蛋白扭曲原肠胚形成（Tsg）。虽然果蝇和脊椎动物只有一个sog/chordin基因，但它们拥有几个rid和tsg的旁系同源物。一个更简单的和可能的祖先的情况下观察到的短胚芽甲虫赤拟谷盗（Tc），其中只有一个TLD和一个tsg基因。在这里，我们表明，在T。castaneum tld是早期BMP信号传导所需的，除了头部区域，并且如所预期的，Tc-tld功能依赖于Tc-sog。相反，Tc-tsg是早期BMP信号传导的所有方面所必需的，并且以Tc-sog独立的方式起作用。为了与果蝇进行比较，我们构建了缺乏所有早期Tsg活性的果蝇胚胎（tsg;：srw双突变体），并表明它们仍然建立了BMP信号梯度。因此，我们的研究结果表明，Tsg蛋白的BMP梯度形成的作用在昆虫进化过程中发生了变化。(C)2010年爱思唯尔公司All rights reserved.
The formation of the BMP gradient which patterns the DV axis in flies and vertebrates requires several extracellular modulators like the inhibitory protein Sog/Chordin, the metalloprotease Tolloid (Tld), which cleaves Sog/Chordin, and the CR domain protein Twisted gastrulation (Tsg). While flies and vertebrates have only one sog/chordin gene they possess several paralogues of rid and tsg. A simpler and probably ancestral situation is observed in the short-germ beetle Tribolium castaneum (Tc), which possesses only one tld and one tsg gene. Here we show that in T. castaneum tld is required for early BMP signalling except in the head region and Tc-tld function is, as expected, dependent on Tc-sog. In contrast, Tc-tsg is required for all aspects of early BMP signalling and acts in a Tc-sog-independent manner. For comparison with Drosophila melanogaster we constructed fly embryos lacking all early Tsg activity (tsg;:srw double mutants) and show that they still establish a BMP signalling gradient. Thus, our results suggest that the role of Tsg proteins for BMP gradient formation has changed during insect evolution. (C) 2010 Elsevier Inc. All rights reserved.