Isolation and characterization of autoreactive T cells in experimental autoimmune encephalomyelitis of the mouse
Isolation and characterization of autoreactive T cells in experimental autoimmune encephalomyelitis of the mouse
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DOI：
10.1006/meth.1996.0053
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Steinman, Lawrence
中科院分区：
文献类型：
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作者：
Brocke, Stefan;Quigley, Laura;Steinman, Lawrence
实验性自身免疫性脑脊髓炎（EAE）可以通过对中枢神经系统自身抗原特异性的T细胞过继转移。各种自身抗原及其衍生肽已被证明是致脑炎T细胞系和克隆的优良刺激物。本文介绍了建立和自身反应性T细胞系和克隆的特性和这些细胞诱导的EAE的研究协议。使用转移EAE模型的基本原理进行了讨论，连同使用在体外培养的T细胞相比，弗氏佐剂为基础的免疫诱导EAE的优点和缺点。
Experimental autoimmune encephalomyelitis (EAE) can be adoptively transferred by T cells that are specific for autoantigens of the central nervous system. A variety of autoantigens and their derived peptides have been shown to be excellent stimulators for encephalitogenic T-cell lines and clones. This article describes protocols for the establishment and characterization of autoreactive T-cell lines and clones and for the study of EAE induced by these cells. The rationale for using transferred EAE models is discussed, together with advantages and disadvantages of using in vitro cultured T cells compared with Freund's adjuvant-based immunization for the induction of EAE.