Antioxidant balance and accumulation of advanced glycation end products after the consumption of standard diets including Maillard reaction products from silver carp peptides.
Antioxidant balance and accumulation of advanced glycation end products after the consumption of standard diets including Maillard reaction products from silver carp peptides.
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DOI：
10.1039/c6fo01183g
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发表时间：
2016-11
期刊：
影响因子：
6.1
通讯作者：
Ye Yao;Kaining Han;S. Dong;M. Zeng;Zunying Liu
中科院分区：
文献类型：
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作者：
Ye Yao;Kaining Han;S. Dong;M. Zeng;Zunying Liu
本研究首次研究了不同加热温度下，以含有银肽-葡萄糖模型美拉德反应产物（Maillard reaction products，MRPs）的饲料喂养链脲佐菌素（STZ）糖尿病C57 BL/6 J小鼠，观察其糖基化终产物（AGEs，包括荧光物质和N-ε-羧甲基赖氨酸（CML））的积累和排泄情况。并分析其体外DPPH和ORAC抗氧化活性与体内氧化应激的关系。MRPs的体外抗氧化活性与其AGEs水平和分子量分布呈正相关。而丙二醛（MDA）和超氧化物歧化酶（SOD）的测定结果则相反，表明较高加热温度的MRPs会加重糖尿病小鼠的氧化应激。血清中CML蓄积加重，但饲料中MRPs的变化对CML在肾脏和肝脏中蓄积几乎没有影响。因此，来自含有肽MRPs的饮食的高AGEs水平对糖尿病小鼠的氧化应激具有负面影响。
The aim of this research was to investigate the accumulation and excretion of AGEs including fluorescent compounds and N-ε-carboxymethyllysine (CML) in streptozotocin-induced diabetes C57BL/6J mice fed on a diet containing Maillard reaction products (MRPs) from a silver carp peptide-glucose model system at different heating temperatures for the first time, and analyze the relationship between their in vitro antioxidant activity based on DPPH and ORAC assays and in vivo oxidative stress. The in vitro antioxidant activity of MRPs positively correlated with their AGEs level and molecular weight distribution. However, the opposite results were found by malondialdehyde (MDA) and super oxide dismutase (SOD) assays that MRPs from a higher heating temperature could aggravate the oxidative stress of diabetic mice. CML accumulation in serum was aggravated, but changes of MRPs in diets hardly affected CML accumulation in the kidney and liver. Therefore, the high AGEs levels from a diet containing peptide MRPs have negative effects on the oxidative stress of diabetic mice.