Microarray analysis reveals overlapping and specific transcriptional responses to different plant hormones in rice
Microarray analysis reveals overlapping and specific transcriptional responses to different plant hormones in rice
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DOI：
10.4161/psb.20910
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发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Jain, Mukesh
中科院分区：
文献类型：
--
作者：
Garg, Rohini;Tyagi, Akhilesh K.;Jain, Mukesh
激素对植物生长发育具有多效作用。这些效应中的许多是通过改变基因表达在分子水平上介导的。本研究利用基因芯片技术，在全基因组水平上研究了植物激素（包括生长素、细胞分裂素、脱落酸、乙烯、水杨酸和茉莉酸）对水稻基因转录的外源影响。我们的分析鉴定了总共4171个基因，这些基因参与了几个生物过程，它们的表达在不同激素的存在下发生了显著的改变。此外，这些基因中有28%在任何两种激素的存在下表现出重叠的转录反应，表明植物激素之间的串扰。此外，我们鉴定了仅显示特定的大肠杆菌特异性应答的基因，其可用作大肠杆菌特异性标记。本研究结果将有助于水稻激素生物学的进一步研究。
Hormones exert pleiotropic effects on plant growth and development throughout the life cycle. many of these effects are mediated at molecular level via altering gene expression. in this study, we investigated the exogenous effect of plant hormones, including auxin, cytokinin, abscisic acid, ethylene, salicylic acid and jasmonic acid, on the transcription of rice genes at whole genome level using microarray. our analysis identified a total of 4171 genes involved in several biological processes, whose expression was altered significantly in the presence of different hormones. Further, 28% of these genes exhibited overlapping transcriptional responses in the presence of any two hormones, indicating crosstalk among plant hormones. in addition, we identified genes showing only a particular hormone-specific response, which can be used as hormone-specific markers. the results of this study will facilitate further studies in hormone biology in rice.