Th17/Tc17 infiltration and associated cytokine gene expression in elicitation phase of allergic contact dermatitis.
Th17/Tc17 infiltration and associated cytokine gene expression in elicitation phase of allergic contact dermatitis.
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DOI：
10.1111/j.1365-2133.2009.09400.x
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发表时间：
2009-12
期刊：
影响因子：
--
通讯作者：
Gaspari AA
中科院分区：
文献类型：
--
作者：
Zhao Y;Balato A;Fishelevich R;Chapoval A;Mann DL;Gaspari AA
Allergic contact dermatitis is a typical delayed-type hypersensitivity to sensitizing haptens mediated by T cells. Th1/Tc1 cells are currently considered to be the primary effectors in allergic contact dermatitis. There is little information concerning the role played in allergic contact dermatitis in humans by Th-17/Tc-17 cells, a recently defined subpopulation of effector T cells. In the present report we attempted to characterize Th-17/Tc-17 cells in the infiltrates of the skin in elicitation phase of allergic contact dermatitis. Th-17 as well as Th1/Th2 cytokine gene expression was examined by semi-quantitative real-time polymerase chain reaction in paired samples of positive patch test biopsies and normal skins from 11 patients allergic to 9 different allergens. The in situ characterization of IL-17-producing cells was carried out using anti-RORC and anti-T cell subset antibodies by double immunofluorescence. Compared to normal paired skins, gene expression of transcription factor for human Th-17 cells, RORC, and Th-17-related cytokines IL-17A, IL-17F and IL-23, was significantly increased in positive patch test biopsies. The mRNA for IFN-γ and IL-4 was also increased. In the dermal infiltrates, about 20 % of the infiltrating cells were IL-17-producing cells as they expressed RORC, and such RORC expressing cells were detected in both CD4+ (~30%) and CD8+ (~20%) subsets. This is the first demonstration of Th-17/Tc-17 cells in the elicitation phase of human allergic contact dermatitis, showing that they are a regular participant in the immunopathology of this common allergic reaction regardless of the nature of the triggering allergens.
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