Expression of the transcription factor Zfp191 during embryonic development in the mouse
Expression of the transcription factor Zfp191 during embryonic development in the mouse
复制标题
DOI：
10.1016/j.gep.2007.11.002
复制
发表时间：
2008-02-01
影响因子：
1.2
通讯作者：
Mallet, Jacques
中科院分区：
文献类型：
--
作者：
Khalfallah, Olfa;Faucon-Biguet, Nicole;Mallet, Jacques
人锌指蛋白191(ZNF191)是一种类似Kruppel的蛋白，可以与广泛存在的TCAT基序特异地相互作用，该基序构成了酪氨酸羟基酶(TH)基因中的HUMTH01微卫星(编码儿茶酚胺合成的限速酶)。已知HUMTH01的等位基因变异对TH基因的表达有定量的沉默作用，并与ZNF191结合的数量和质量的变化相关。该因子已经从骨髓和早幼粒细胞白血病细胞系中分离出来，表明ZNF191也在造血中发挥作用。因此，ZNF191可能参与了与不同功能相关的多个基因的调控。此外，Zfp191(ZNF191的小鼠同源物)基因缺失的小鼠被证明发育严重迟缓，大约在胚胎7.5天死亡。为了进一步了解Zfp191的生物学功能，我们分析了Zfp191在小鼠发育过程中的定位。在胚胎发育早期，外胚层、内胚层、中胚层和胚外组织中均有表达。特别是，Zfp191在发育中的中枢神经系统中被观察到。有趣的是，它的表达水平在增殖区如脑室下区显著。Zfp191在发育过程中的表达模式可以解释Zfp191(-1-)胚胎的表型特征。(C)2007 Elsevier B.V.保留所有权利。
The human zinc finger protein 191 (ZNF191) is a Kruppel-like protein and can specifically interact with the widespread TCAT motif which constitutes the HUMTH01 microsatellite in the tyrosine hydroxylase (TH) gene (encoding the rate-limiting enzyme in the synthesis of catecholamines). Allelic variations of HUMTH01 are known to have a quantitative silencing effect on TH gene expression and to correlate with quantitative and qualitative changes in the binding by ZNF191. This factor has been isolated from bone marrow and promyelocytic leukemia cell lines indicating that ZNF191 also plays a role in hematopoiesis. Thus, ZNF191 could participate in the regulation of several genes implicated in different functions. Moreover, mice that are deficient in Zfp191, the murine homologue of ZNF191, have been shown to be severely retarded in development and to die approximately at embryonic day 7.5. In order to gain further insight into its biological functions, we have analysed the localisation of Zfp191 throughout mouse development. Expression was detected early during embryogenesis in ectodermal, endodermal, mesodermal and extra-embryonic tissues. In particular, Zfp191 was observed in the developing central nervous system. Interestingly, its expression levels were prominent in areas of proliferation such as the subventricular zone. Zfp191 expression pattern during development can account for the phenotypic features of Zfp191(-1-) embryos. (c) 2007 Elsevier B.V. All rights reserved.