Regulation of spine calcium dynamics by rapid spine motility
Regulation of spine calcium dynamics by rapid spine motility
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DOI：
10.1523/jneurosci.20-22-08262.2000
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发表时间：
2000-11-15
影响因子：
5.3
通讯作者：
Yuste, R
中科院分区：
文献类型：
--
作者：
Majewska, A;Tashiro, A;Yuste, R
树突棘接收中枢神经系统中大部分的兴奋性输入并分隔钙。刺也会经历快速的形态变化，尽管这种运动的功能仍不清楚。我们通过增强型绿色荧光蛋白的双光子光漂白和小鼠视觉皮层切片中 2/3 层锥体神经元的动作电位诱发瞬变的钙成像，研究了脊柱运动对脊柱钙动力学的影响。脊柱运动过程中脊柱颈部的伸长或缩回改变了脊柱和树突之间的扩散耦合，并显着改变了脊柱中的钙衰变动力学。我们的结果表明，脊柱分隔钙的能力在不断变化。
Dendritic spines receive most excitatory inputs in the CNS and compartmentalize calcium. Spines also undergo rapid morphological changes, although the function of this motility is still unclear. We have investigated the effect of spine movement on spine calcium dynamics with two-photon photobleaching of enhanced green fluorescent protein and calcium imaging of action potential-elicited transients in spines from layer 2/3 pyramidal neurons in mouse visual cortex slices. The elongation or retraction of the spine neck during spine motility alters the diffusional coupling between spine and dendrite and significantly changes calcium decay kinetics in spines. Our results demonstrate that the spine's ability to compartmentalize calcium is constantly changing.