Low-intensity pulsed ultrasound stimulation for mandibular condyle osteoarthritis lesions in rats
Low-intensity pulsed ultrasound stimulation for mandibular condyle osteoarthritis lesions in rats
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DOI：
10.1111/odi.12798
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发表时间：
2018-05-01
期刊：
影响因子：
3.8
通讯作者：
Ono, T.
中科院分区：
文献类型：
--
作者：
Arita, A. Kanaguchi;Yonemitsu, I.;Ono, T.
本研究评价了低强度脉冲超声对成年大鼠颞下颌关节骨关节炎的作用。材料与方法24只成年大鼠颞下颌关节用低剂量单碘乙酸注射诱导骨关节炎样病变。将大鼠分为4组：对照组和单碘乙酸组，分别于第12周注射造影剂和单碘乙酸，观察至第20周;低强度脉冲超声组和单碘乙酸+低强度脉冲超声组，分别于第12周注射造影剂和单碘乙酸，并于第16 ~ 20周进行低强度脉冲超声。每周用微型计算机断层扫描评估髁突骨密度、骨矿含量和骨体积。结果20周时，单碘乙酸+低强度脉冲超声组骨密度、骨矿含量和骨体积均显著高于单碘乙酸组，但仍低于其他两组。组织学和免疫组织化学分析显示，单碘乙酸+低强度脉冲超声组软骨细胞数量明显增加，基质金属蛋白酶13免疫阳性细胞数量明显少于单碘乙酸组。
ObjectiveThis study evaluated low-intensity pulsed ultrasound effects for temporomandibular joint osteoarthritis in adult rats.Material and methodsOsteoarthritis-like lesions were induced in 24 adult rats' temporomandibular joints with low-dose mono-iodoacetate injections. The rats were divided into four groups: control and mono-iodoacetate groups, injected with contrast media and mono-iodoacetate, respectively, at 12weeks and observed until 20weeks; and low-intensity pulsed ultrasound and mono-iodoacetate + low-intensity pulsed ultrasound groups, injected with contrast media and mono-iodoacetate, respectively, at 12weeks with low-intensity pulsed ultrasound performed from 16 to 20weeks. Condylar bone mineral density, bone mineral content and bone volume were evaluated weekly with microcomputed tomography. Histological and immunohistochemical staining for matrix metalloproteinases-13 was performed at 20weeks.ResultsAt 20weeks, the mono-iodoacetate + low-intensity pulsed ultrasound group showed significantly higher bone mineral density, bone mineral content and bone volume than the mono-iodoacetate group; however, these values remained lower than those in the other two groups. On histological and immunohistochemical analysis, the chondrocytes were increased, and fewer matrix metalloproteinases-13 immunopositive cells were identified in the mono-iodoacetate + low-intensity pulsed ultrasound group than mono-iodoacetate group.ConclusionsLow-intensity pulsed ultrasound for 2weeks may have therapeutic potential for treating temporomandibular joint osteoarthritis lesions.