Regional incorporation and site-specific differentiation of striatal precursors transplanted to the embryonic forebrain ventricle
Regional incorporation and site-specific differentiation of striatal precursors transplanted to the embryonic forebrain ventricle
复制标题
DOI：
10.1016/0896-6273(95)90006-3
复制
发表时间：
1995-12-01
期刊：
影响因子：
16.2
通讯作者：
Bjorklund, A
中科院分区：
文献类型：
--
作者：
Campbell, K;Olsson, M;Bjorklund, A
在跨物种移植模型中，研究了来自E13.5-E14外侧或内侧神经节隆起(分别为LGE和MGE)或E12腹侧中脑(Vm)的神经前体细胞的发育潜力。注射入E15大鼠前脑室后，小鼠LGE前体细胞(不同于MGE或Vm)一直整合到宿主纹状体，表达纹状体投射神经元特有的神经化学表型和轴突投射。此外，LGE和MGE前体细胞广泛整合到不同的前脑和中脑结构中，而更多来自尾部的VM细胞主要局限于中脑结构。这些结果表明，许多LGE前体在位置上被指定为纹状体结合，而一部分也具有更大的潜力，反映在脑室注射后更广泛的整合中。
The developmental potential of neural progenitors derived from the E13.5-E14 lateral or medial ganglionic eminences (LGE and MGE, respectively) or the E12 ventral mesencephalon (VM) was examined in a cross-species transplantation model. After injection into the E15 rat forebrain ventricle, mouse LGE progenitors (unlike those of the MGE or VM) were consistently integrated into the host striatum, expressing neurochemical phenotypes and axonal projections characteristic of striatal projection neurons. Additionally, both LGE and MGE precursors displayed widespread incorporation into distinct forebrain and midbrain structures, whereas the more caudally derived VM cells were largely confined to midbrain structures. These results suggest that many LGE precursors are positionally specified for striatal incorporation, while a portion also possess greater potential reflected in more widespread integration following intraventricular injection.