Effects on bone regeneration when collagen model polypeptides are combined with various sizes of alpha-tricalcium phosphate particles.
Effects on bone regeneration when collagen model polypeptides are combined with various sizes of alpha-tricalcium phosphate particles.
复制标题
DOI：
10.4012/dmj.2011-126
复制
发表时间：
2011-11
影响因子：
2.5
通讯作者：
Kana Sakai;Y. Hashimoto;S. Baba;Aki Nishiura;N. Matsumoto
中科院分区：
文献类型：
--
作者：
Kana Sakai;Y. Hashimoto;S. Baba;Aki Nishiura;N. Matsumoto
我们评价了在小型猪颅骨缺损模型中组合由Pro-Hyp-Gly [poly（PHG）]序列组成的合成胶原模型多肽和具有不同中值尺寸（大：580.8 μm;小：136.2 μm;或大小混合：499.3 μm）的α-磷酸三钙（α-TCP）颗粒对骨形成的影响。定量图像分析显示，植入后4周，各α-TCP组的新生骨体积密度（VD）均显著高于poly（PHG）对照组，其中混合组的VD最高。组织学评估显示，小α-TCP颗粒在8周时几乎完全降解。12周时，所有尺寸的α-TCP颗粒完全降解，并观察到板层骨的重建。目前的研究结果表明，颗粒大小可能会影响骨形成的成功缺陷。
We evaluated the effects on bone formation of combining synthesized collagen model polypeptides consisting of a Pro-Hyp-Gly [poly(PHG)] sequence and alpha-tricalcium phosphate (α-TCP) particles with various median sizes (large: 580.8 μm; small: 136.2 μm; or large and small mixed: 499.3 μm) in a skull defect model in mini-pigs. Quantitative image analyses for the volume density (VD) of new bone revealed that the VD in each α-TCP group was significantly higher than that in the poly(PHG) control group, with the mixed group showing the highest VD among all the groups at 4 weeks after implantation. Histological assessments revealed that the small α-TCP particles were almost completely degraded at 8 weeks. At 12 weeks, all sizes of α-TCP particles were completely degraded and remodeling of the lamellar bone was observed. The present findings suggest that particle size may influence the success of bone formation in defects.