Adenoviral gene transfer into isolated pancreatic islets.
Adenoviral gene transfer into isolated pancreatic islets.
复制标题
腺病毒基因转移到分离的胰岛中。
DOI：
10.1007/978-1-60327-378-7_8
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Ozcan,Sabire
中科院分区：
文献类型：
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作者：
Muniappan,Latha;Ozcan,Sabire
胰岛内的β细胞负责产生胰岛素，胰岛素是维持正常血糖所需的肽激素。建立有效的基因转移到胰岛中对于研究胰岛素和胰高血糖素的产生和分泌以及用于治疗糖尿病的基因治疗目的是非常重要的。我们在这里详细描述了腺病毒基因转移到孤立的小鼠胰岛的协议。使用重组腺病毒将有效的基因转移到胰岛中可以以10的感染复数（MOI）实现。然而，如果胰岛不分散，腺病毒基因转移仅限于胰岛周围的细胞，其代表啮齿动物中产生胰高血糖素的α细胞。用EGTA分散胰岛增加了基因转移到胰岛核心内的细胞中的效率，所述胰岛核心由产生胰岛素的β细胞组成。
The beta cells within the pancreatic islets are responsible for production of insulin, a peptide hormone required for maintaining normoglycemia. The establishment of efficient gene transfer into pancreatic islets is very important for studies of insulin and glucagon production and secretion, as well as for gene therapy purposes for the treatment of diabetes. We describe here in detail a protocol for adenoviral gene transfer into isolated mouse islets of the pancreas. Effective gene transfer into pancreatic islets using recombinant adenoviruses can be achieved with a multiplicity of infection (MOI) of 10. However, if the islets are not dispersed, adenoviral gene transfer is limited only to the cells on the periphery of the islets, which represent the glucagon-producing alpha cells in rodents. Dispersion of pancreatic islets with EGTA increases the efficiency of gene transfer into the cells within the core of the islets, which consist of insulin-producing beta cells.