Disruption of three polyamine uptake transporter genes in rice by CRISPR/Cas9 gene editing confers tolerance to herbicide paraquat.
Disruption of three polyamine uptake transporter genes in rice by CRISPR/Cas9 gene editing confers tolerance to herbicide paraquat.
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DOI：
10.1007/s42994-022-00075-4
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发表时间：
2022-06
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杂草是主要的生物限制因素，可能导致农作物产量严重损失。除草剂技术作为最具成本效益的杂草控制措施已被农民广泛使用，迫切需要开发新的策略来提高植物对除草剂的耐受性。基于 CRISPR/Cas9 的基因组编辑工具已被用于与作物改良农业技术相关的多种应用。在这里，我们鉴定了水稻中与拟南芥 AtRMV1 同源的三个多胺摄取转运蛋白 (PUT) 基因。我们成功证明了 OsPUT1/2/3 的 CRISPR/Cas9 靶向诱变极大地提高了水稻的百草枯抗性，且没有明显的产量损失。因此，操纵这些基因座对于未来生产具有改善的除草剂抗性的非转基因水稻具有重要价值。在线版本包含可在 10.1007/s42994-022-00075-4 获取的补充材料。
Weeds are a major biotic constraint that can cause dramatic crop production losses. Herbicide technology has been widely used by farmers as the most cost-effective weed control measure, and development of new strategy to improve herbicide tolerance in plants is urgently needed. The CRISPR/Cas9-based genome editing tool has been used in diverse applications related to agricultural technology for crop improvement. Here we identified three polyamine uptake transporter (PUT) genes in rice that are homologous to the Arabidopsis AtRMV1. We successfully demonstrate that CRISPR/Cas9-targeted mutagenesis of OsPUT1/2/3 greatly improves paraquat resistance in rice without obvious yield penalty. Therefore, manipulation of these loci could be valuable for producing transgene-free rice with improved herbicide resistance in future. The online version contains supplementary material available at 10.1007/s42994-022-00075-4.