Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition
Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition
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DOI：
10.1128/aem.66.12.5488-5491.2000
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发表时间：
2000-12-01
影响因子：
4.4
通讯作者：
Bailey, MJ
中科院分区：
文献类型：
--
作者：
Griffiths, RI;Whiteley, AS;Bailey, MJ
描述了从环境样品中提取总核酸的快速方案。该方法有利于伴随着从一个单一的提取，变性梯度凝胶电泳微生物群落分析的活性成分（rRNA衍生）从总的细菌多样性（核糖体DNA衍生）的视野下建立草原土壤的PCR和逆转录-PCR扩增的微生物16 S rRNA多样性的评估。
A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction, Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.