Cadherin is required for dendritic morphogenesis and synaptic terminal organization of retinal horizontal cells
Cadherin is required for dendritic morphogenesis and synaptic terminal organization of retinal horizontal cells
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DOI：
10.1242/dev.02566
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发表时间：
2006-10-15
期刊：
影响因子：
4.6
通讯作者：
Nakagawa, Shinichi
中科院分区：
文献类型：
--
作者：
Tanabe, Koji;Takahashi, Yoshiko;Nakagawa, Shinichi
神经元的树突形态为其生理特性提供了结构基础，并以细胞类型依赖的方式受到精确调控。利用一种独特的转座子介导的基因转移系统，能够有条件地和细胞类型特异性地表达外源基因，我们研究了钙粘附素在发育中的鸡视网膜水平细胞树突状形态发生中的作用。我们首先通过过度表达膜靶向EGFP来可视化单个水平细胞，并证实水平细胞有三种亚型，其树突状终末投射到外丛状层中不同的突触位置。显性-负性钙粘附素的表达减少了树突野的大小，并扰乱了树突状突起在感光细胞上的终止。钙粘附素的阻断也损害了突触后标志物GluR4在锥体椎弓根的积累。因此，我们提供了体内证据，证明在脊椎动物视网膜中，钙粘附素是水平细胞的树突形态发生和随后与感光细胞形成突触所必需的。
Dendrite morphology of neurons provides a structural basis for their physiological characteristics, and is precisely regulated in a cell type-dependent manner. Using a unique transposon-mediated gene transfer system that enables conditional and cell-type specific expression of exogenous genes, we investigated the role of cadherin on dendritic morphogenesis of horizontal cells in the developing chicken retina. We first visualized single horizontal cells by overexpressing membrane-targeted EGFP, and confirmed that there were three subtypes of horizontal cells, the dendritic terminals of which projected to distinct synaptic sites in the outer plexiform layer. Expression of a dominant-negative cadherin decreased the dendritic field size, and perturbed the termination of dendritic processes onto the photoreceptor cells. The cadherin blockade also impaired the accumulation of GluR4, a postsynaptic marker, at the cone pedicles. We thus provide in vivo evidence that cadherin is required for dendrite morphogenesis of horizontal cells and subsequent synapse formation with photoreceptor cells in the vertebrate retina.