Comparison of Genetic and Physiological Properties of Salmonella enterica Isolates from Chickens Reveals One Major Difference Between Serovar Kentucky and Other Serovars: Response to Acid
Comparison of Genetic and Physiological Properties of Salmonella enterica Isolates from Chickens Reveals One Major Difference Between Serovar Kentucky and Other Serovars: Response to Acid
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DOI：
10.1089/fpd.2008.0144
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发表时间：
2009-05-01
影响因子：
2.8
通讯作者：
Kniel, Kalmia E.
中科院分区：
文献类型：
--
作者：
Joerger, Rolf D.;Sartori, Casey A.;Kniel, Kalmia E.
由于未知的原因，肯塔基州肠道沙门氏菌已成为美国最常从鸡和鸡尸体中分离出的血清型。为了确定可能导致这种现象的性状，将30个血清型肯塔基州鸡分离株的遗传和生理特征与属于一系列其他S.肠道血清型。通过PCR在血清型肯塔基州分离株中检测到大多数已知的沙门氏菌毒力基因，但未发现cdtB、spvB、spvC和pefA基因。在涉及鸡胚肝细胞的体外试验中，血清型肯塔基州分离株与非肯塔基州分离株一样具有侵袭性，但与人HCT-8细胞孵育时，侵袭性低于肠杆菌、姆班达基和鼠伤寒分离株。对来自血清型肯塔基州和来自肠杆菌、Hadar、Mbandaka、森夫滕贝格、鼠伤寒和沃辛顿分离株的生长、生物膜形成和应激存活数据进行统计学比较，结果显示无差异或仅与少数血清型存在差异;然而，3个数据集不同。这些数据集包括在用乙酸调节至pH 5.5的胰蛋白酶大豆肉汤（TSB）中生长的培养物的OD（600）值，以及在TSB（pH 7）或TSB（用乙酸调节至pH 5.5，然后转移至TSB（用HCl调节至pH 2.5）中）中生长的细胞的存活计数。最值得注意的是，乙酸预暴露肯塔基州分离株的log（10）减少值为3.1，
For unknown reasons, Salmonella enterica Kentucky has become the serovar most frequently isolated from chickens and chicken carcasses in the United States. In an attempt to identify traits that may underlie this phenomenon, genetic and physiological features of 30 serovar Kentucky chicken isolates were compared with those of chicken isolates belonging to a range of other S. enterica serovars. Most of the well-known Salmonella virulence genes were detected in the serovar Kentucky isolates by PCR, but the cdtB, spvB, spvC, and pefA genes were not found. The serovar Kentucky isolates were as invasive as the non-Kentucky isolates in in vitro assays involving chicken embryo hepatocytes, but were less invasive than the Enteritidis, Mbandaki, and Typhimurium isolates when incubated with human HCT-8 cells. Statistical comparison of growth, biofilm formation, and stress survival data from the serovar Kentucky and those from the serovar Enteritidis, Hadar, Mbandaka, Senftenberg, Typhimurium, and Worthington isolates demonstrated either no differences or differences with only a few of the serovars; however, three data sets were different. These data sets included the OD(600) values of cultures grown in tryptic soy broth (TSB) adjusted to pH 5.5 with acetic acid and survival counts of cells grown in either TSB pH 7 or TSB adjusted to pH 5.5 with acetic acid and then transferred into TSB adjusted to pH 2.5 with HCl. Most notable was the log(10) reduction for acetic acid pre-exposed Kentucky isolates of 3.1 versus