Polysialic acid immobilized on silanized glass surfaces: a test case for its use as a biomaterial for nerve regeneration
Polysialic acid immobilized on silanized glass surfaces: a test case for its use as a biomaterial for nerve regeneration
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DOI：
10.1007/s10856-009-3981-0
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发表时间：
2010-01
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影响因子：
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通讯作者：
Stephanie Steinhaus;Yvonne Stark;S. Bruns;Y. Haile;T. Scheper;C. Grothe;P. Behrens
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文献类型：
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作者：
Stephanie Steinhaus;Yvonne Stark;S. Bruns;Y. Haile;T. Scheper;C. Grothe;P. Behrens
研究了聚唾液酸（polySia）在玻璃基质上的固定化，以研究这种多糖作为一种新型的、生物相容性和生物可吸收的组织工程材料的适用性，特别是在神经再生方面的应用。PolySia是α-2,8链唾液酸的均聚物，参与神经细胞粘附分子（NCAM）的翻译后修饰。聚sia的降解是可以控制的，这使其成为组织工程中涂层和支架结构的有趣材料。在这里，我们描述了通过环氧硅烷连接剂在玻璃表面上固定聚sia。虽然玻璃表面实际上不会用于神经再生支架，但它们提供了一种简单有效的方法来测试各种方法来研究固定化息肉。采用接触角法对修饰后的表面进行了研究，并采用硫代巴比妥酸法和特异的polySia- elisa法检测了固定化polySia的数量。通过细胞黏附和细胞活力测试来评估聚西亚修饰表面与永生化雪旺细胞之间的相互作用。结果表明，聚sia可以通过环氧硅烷连接剂固定在玻璃表面，并且表面结合的聚sia对雪旺细胞没有毒性作用。因此，作为脊椎动物发育的关键物质和培养雪旺细胞的良好底物，它在神经引导管中用于治疗周围神经损伤提供了有趣的特性。
The immobilization of polysialic acid (polySia) on glass substrates has been investigated with regard to the applicability of this polysaccharide as a novel, biocompatible and bioresorbable material for tissue engineering, especially with regard to its use in nerve regeneration. PolySia, a homopolymer of α-2,8-linked sialic acid, is involved in post-translational modification of the neural cell adhesion molecule (NCAM). The degradation of polySia can be controlled which makes it an interesting material for coating and for scaffold construction in tissue engineering. Here, we describe the immobilization of polySia on glass surfaces via an epoxysilane linker. Whereas glass surfaces will not actually be used in nerve regeneration scaffolds, they provide a simple and efficient means for testing various methods for the investigation of immobilized polySia. The modified surfaces were investigated with contact angle measurements and the quantity of immobilized polySia was examined by the thiobarbituric acid assay and a specific polySia-ELISA. The interactions between the polySia-modified surface and immortalized Schwann cells were evaluated via cell adhesion and cell viability assays. The results show that polySia can be immobilized on glass surfaces via the epoxysilane linker and that surface-bound polySia has no toxic effects on Schwann cells. Therefore, as a key substance in the development of vertebrates and as a favourable substrate for the cultivation of Schwann cells, it offers interesting features for the use in nerve guidance tubes for treatment of peripheral nerve injuries.