Magnetic resonance imaging correlated with the histopathological effect of Pd-bacteriopheophorbide (Tookad) photodynamic therapy on the normal canine prostate gland
Magnetic resonance imaging correlated with the histopathological effect of Pd-bacteriopheophorbide (Tookad) photodynamic therapy on the normal canine prostate gland
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DOI：
10.1002/lsm.20375
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发表时间：
2006-08-01
影响因子：
2.4
通讯作者：
Hetzel, Fred W.
中科院分区：
文献类型：
--
作者：
Huang, Zheng;Haider, Masoom A.;Hetzel, Fred W.
背景和目的：为了确定最佳的磁共振成像（MRI）的方法来评估光动力疗法（PDT）诱导的组织病理学反应，在prostate.Study设计/材料和方法：剖腹手术进行了五个健康的狗。将圆柱形扩散器置于前列腺中，以在150 mW/cm和763 nm下递送50-300 J/cm的光，以激活IV注射的Tookad（1 mg/kg b.w.）。在PDT前、PDT后2天、7天和1个月获得快速自旋回波（FSE）T2加权、对比增强后T1（CE-T1）和弥散加权图像（DWI）。在PDT后7天（n = 4）和1个月（n=1）进行放射学-组织病理学相关性。信号变化和表观扩散系数（ADC）mapping的定性评估performed.Results：在PDT后2或7天，PDT诱导的出血性坏死和CE-T1图像上的未增强区域之间存在良好的空间相关性。有一个快速和持续增强的边缘，对应于水肿和炎症。所有病例FSE T2和DWI均显示信号改变，但不能明确定义坏死。在1个月，这是很难相关的MR图像的组织病理学变化，因为它们代表了一个混合物的坏死和发展中的纤维化，从而导致混合信号强度和少划定对比enhancement.Conclusions：在PDT后7天，钆DTPA对比增强MRI是上级DWI和T2成像在评估Tookad PDT诱导的组织坏死在正常犬前列腺的边界。
Background and Objective: To determine the optimal magnetic resonance imaging (MRI) methodology to assess photodynamic therapy (PDT)-induced histopathological responses in the prostate.Study Design/Materials and Methods: Laparotomy was performed in five healthy dogs. Cylindrical diffuser was placed in the prostates to deliver light of 50-300 J/cm at 150 mW/cm and 763 nm to activate IV-injected Tookad (1 mg/kg b.w.). Fast spin echo (FSE) T2-weighted, post-contrast-enhanced T1-(CE-T1) and diffusion weighted images (DWI) were obtained pre- and 2 days, 7 days, and 1 month post-PDT. Radiological-histopathological correlation was performed at 7 days (n = 4) and 1 month (n=1) after PDT. A qualitative assessment of signal changes and apparent diffusion coefficient (ADC) mapping was performed.Results: At 2 or 7 days post-PDT, there was good spatial correlation between PDT-induced hemorrhagic necrosis and unenhanced regions on CE-T1 images. There was a rapidly and persistently enhancing rim corresponding to edema and inflammation. FSE T2 and DWI showed altered signal but did not clearly define necrosis in all cases. At 1 month, it was hard to correlate MR images to histopathologic changes as they represented a mixture of necrosis and developing fibrosis, which led to a mixed signal intensity and less demarcated contrast enhancement.Conclusions: At 7 days after PDT, gadolinium DTPA contrast-enhanced MRI is superior to DWI and T2 imaging in assessing the boundary of Tookad PDT-induced tissue necrosis in the normal canine prostate.