Fibroblast levels are increased in chronic rhinosinusitis with nasal polyps and are associated with worse subjective disease severity
Fibroblast levels are increased in chronic rhinosinusitis with nasal polyps and are associated with worse subjective disease severity
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DOI：
10.1002/alr.21636
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发表时间：
2016-02-01
影响因子：
6.4
通讯作者：
Mulligan, Jennifer K.
中科院分区：
文献类型：
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作者：
Carroll, William W.;O'Connell, Brendan P.;Mulligan, Jennifer K.
成纤维细胞参与慢性鼻窦炎（CRS）的组织重塑和炎症细胞的募集。CRS亚型中的成纤维细胞群体仍未定量。本研究的目的是测量CRS亚型中的成纤维细胞群体，并调查成纤维细胞和疾病severity.MethodsPatients之间的关联，接受鼻内窥镜手术（ESS）的CRS前瞻性招募从2011年1月至2014年12月。对照受试者包括因非炎性疾病（如脑脊液漏修补术或非激素分泌型垂体瘤）接受内镜手术的患者。患者在手术前完成了22项鼻腔鼻窦结局测试（SNOT-22）问卷。在手术过程中采集血液和组织活检。鼻窦成纤维细胞的百分比通过流式细胞仪测定选择成纤维细胞特异性蛋白（FSP）阳性和粘蛋白1（MUC 1）阴性cells.ResultsA共69例患者参加：对照组（n = 24），CRS无鼻息肉（CRSsNP）（n = 13），CRS鼻息肉（CRSwNP）（n = 22），和过敏性真菌性鼻窦炎（AFRS）（n = 10）。CRSwNP患者的成纤维细胞显著多于对照（p < 0.001）和CRSsNP（p < 0.01）。与对照（p < 0.0001）、CRSsNP（p <0.0001）和CRSwNP（p < 0.05）相比，AFRS患者具有最多的成纤维细胞。特应性和哮喘与CRSwNP中成纤维细胞增加无关（分别为p = 0.21，p = 0.26）。增加的成纤维细胞与主观疾病严重程度相关，如CRSwNP（p = 0.003）和AFRS（p = 0.048）的SN 0 T-22所测量的。CRSwNP和AFRS患者成纤维细胞增加与生活质量下降相关。
BackgroundFibroblasts are implicated in tissue remodeling and recruitment of inflammatory cells in chronic rhinosinusitis (CRS). Populations of fibroblasts remain unquantified in CRS subtypes. The objectives of this study were to measure fibroblast populations in subtypes of CRS, and to investigate the association between fibroblasts and disease severity.MethodsPatients undergoing endoscopic sinus surgery (ESS) for CRS were prospectively enrolled from January 2011 to December 2014. Control subjects included patients undergoing endoscopic surgery for non-inflammatory conditions such as cerebrospinal fluid leak repair or non-hormone-secreting pituitary tumors. Patients completed 22-item Sino-Nasal Outcome Test (SNOT-22) questionnaires prior to surgery. Blood and tissue biopsies were taken during surgery. Percent of sinonasal fibroblasts was determined via flow cytometry by selecting fibroblast-specific protein (FSP)-positive and Mucin 1 (MUC1)-negative cells.ResultsA total of 69 patients were enrolled: control (n = 24), CRS without nasal polyps (CRSsNP) (n = 13), CRS with nasal polyps (CRSwNP) (n = 22), and allergic fungal rhinosinusitis (AFRS) (n = 10). Patients with CRSwNP had significantly more fibroblasts than both control (p < 0.001) and CRSsNP (p < 0.01). Patients with AFRS had the most fibroblasts when compared to control (p < 0.0001), CRSsNP (p < 0.0001), and CRSwNP (p < 0.05). Atopy and asthma were not associated with increased fibroblasts in CRSwNP (p = 0.21, p = 0.26, respectively). Increased fibroblasts correlated with subjective disease severity as measured by SNOT-22 for CRSwNP (p = 0.003) and AFRS (p = 0.048).ConclusionSinonasal fibroblasts are increased in CRSwNP and AFRS compared to control and CRSsNP. Increased fibroblasts correlated with worse quality of life in CRSwNP and AFRS.