Photoinduced Changes in Electrical Potentials and H+ Activities of the Chloroplast, Cytoplasm, and Vacuole of Phaeoceros laevis
Photoinduced Changes in Electrical Potentials and H+ Activities of the Chloroplast, Cytoplasm, and Vacuole of Phaeoceros laevis
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光致棕毛藻叶绿体、细胞质和液泡的电势和 H 活性变化
DOI：
10.1007/978-3-642-65986-7_27
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
R. Davis
中科院分区：
文献类型：
--
作者：
R. Davis
越来越清楚的是，在绿色植物细胞中，H+离子在光诱导的膜电位变化和光磷酸化过程中都发挥着重要的作用。膜电位在明暗(L-D)和暗-暗(D-L)变化后的瞬变被认为依赖于穿过叶绿体类囊体膜的电子流动和离子运动(可能是H+)(L和帕拉吉，1969)。Kitasato(1968)和Spswick(1972)曾考虑过Nitella中的电生H+泵。根据Mitchell(1966)的化学渗透假说，有证据表明H+参与了光合磷酸化。然而，关于光诱导完整叶绿体中H+浓度变化的程度以及这些变化对膜电位的可能影响的了解很少。也没有任何直接的实验证据将细胞质或液泡pH的变化与光诱导的膜电位变化联系起来。因此，本研究的目的是用微电极测量环境中叶绿体以及细胞质和液泡的pH值和电势。
It is becoming increasingly clear that in green plant cells H+ions play an important role both in photoinduced changes of the membrane potential and in photophosphorylation. Transients of the membrane potential after light-dark (L-D) and dark-light (D-L) changes are thought to be dependent on electron flow and ion movements (possibly H+) across the chloroplast thylakoid membranes (Lüttge and Pallaghy, 1969). Kitasato (1968) and Spanswick (1972) have considered electrogenic H+pumps inNitella. There is evidence with broken chloroplasts (HIND and Jagendorf, 1965) for involvement of H+in photophosphorylation in accordance with the chemi-osmotic hypothesis of Mitchell (1966). However, knowledge of the extent of light-induced changes in H+concentration in intact chloroplasts and the possible effects of these changes on the membrane potential is meagre. Nor is there any direct experimental evidence linking changes in cytoplasmic or vacuolar pH to photoinduced changes in the membrane potential. Therefore, the purpose of the present study was to make measurements with microelectrodes of the pH and electrical potential of chloroplastsin situand, as well, of the cytoplasm and vacuole.