Adiponectin-induced dilation of isolated porcine retinal arterioles via production of nitric oxide from endothelial cells.
Adiponectin-induced dilation of isolated porcine retinal arterioles via production of nitric oxide from endothelial cells.
复制标题
脂联素通过内皮细胞产生一氧化氮诱导分离的猪视网膜小动脉扩张。
DOI：
10.1167/iovs.13-11756
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Omae T
中科院分区：
文献类型：
--
作者：
2. Kamiya T;Nagaoka T*;Omae T;Yoshioka T;Ono S;Tanano I;Yoshida A.;Omae T
目的。脂联素是由脂肪细胞分泌的一种重要的脂肪细胞因子，通过脂联素受体（adipoR）对血管组织具有抗炎和动脉粥样硬化保护作用。然而，脂联素在视网膜微循环中的作用尚不清楚。研究了脂联素在猪视网膜小动脉血管舒张作用中的直接作用及其机制。方法：在体外实验中，分离猪视网膜小动脉（内径60-90 μm），插管加压（55 cmH 2 O）。结果：脂联素作用下视网膜小动脉呈剂量依赖性扩张（0.125 ~ 7.5 μg/mL）；去内皮后血管舒张明显减弱。N - g -硝基- l -精氨酸甲酯（一种一氧化氮合酶抑制剂），1h - 1,2,4 -oxadia-zolo [4,3 -a]喹诺沙林-1- 1（一种可溶性guananyyl环化酶抑制剂），但wortmannin（一种磷脂酰肌醇3-激酶抑制剂）对脂联素诱导的血管舒张的抑制作用与剥脱作用相当。用活化蛋白激酶（AMPK）抑制剂化合物C预处理，部分但显著地降低了血管舒张。GW6471（一种过氧化物酶体增殖物激活受体阻滞剂）对脂联素的血管舒张作用没有明显的抑制作用。结论：脂联素主要引起视网膜小动脉内皮依赖性扩张。内皮依赖性血管舒张可能是由脂联素引起的，由NO通过激活胍基环化酶而部分依赖于AMPK活性。了解脂联素对视网膜血管系统的影响可能有助于改善视网膜血管疾病的潜在治疗方法，特别是2型糖尿病患者的糖尿病视网膜病变。
Purpose.: Adiponectin, an important adipocytokine secreted by adipocytes, has anti-inflammatory and atheroprotective effects on vascular tissue via the adiponectin receptor (adipoR). However, the action of adiponectin in the retinal microcirculation is unknown. We examined the direct effect and underlying mechanism of the vasomotor action of adiponectin in porcine retinal arterioles.Methods.: Porcine retinal arterioles (internal diameter, 60–90 μm) were isolated, cannulated, and pressurized (55 cmH 2 O) without flow in this in vitro study. Videomicroscopic techniques were used to record changes in diameter in response to adiponectin.Results.: The retinal arterioles dilated in a dose-dependent (0.125–7.5 μg/mL) manner in response to adiponectin. The vasodilation decreased significantly after removal of the endothelium. N G-nitro-L-arginine methyl ester (a nitric oxide [NO] synthase inhibitor), 1H-1, 2, 4-oxadia-zolo [4, 3-a] quinoxalin-1-one (a soluble guanylyl cyclase inhibitor), but not wortmannin (a phosphatidylinositol 3-kinase inhibitor) inhibited the effect of adiponectin-induced vasodilation comparable with that of denudation. Pretreatment with compound C, an activated protein kinase (AMPK) inhibitor, partially but significantly reduced vasodilation. Incubation with GW6471, a peroxisome proliferator-activated receptor blocker, did not significantly inhibit vasodilation by adiponectin. AdipoR1 and adipoR2 immunoreactions were observed in the endothelium of retinal arterioles.Conclusions.: Adiponectin elicits mainly endothelium-dependent dilation of the retinal arterioles. Endothelium-dependent vasodilation likely induced by adiponectin results from NO via activation of guanylyl cyclase that is partially dependent on AMPK activity. Understanding the effect of adiponectin on the retinal vasculature may help improve potential therapies for retinal vascular disorders, especially diabetic retinopathy in patients with type 2 diabetes mellitus.
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影响因子：
37.8
作者：
Okamoto, Y;Kihara, S;Matsuzawa, Y
通讯作者：
Matsuzawa, Y
影响因子：
3.3
作者：
Rubin, LJ;Magliola, L;Hale, CC
通讯作者：
Hale, CC
影响因子：
--
作者：
KUO, L;DAVIS, MJ;CHILIAN, WM
通讯作者：
CHILIAN, WM
影响因子：
3.5
作者：
Matsuda, M;Kawasaki, F;Kaku, K
通讯作者：
Kaku, K
吡格列酮是一种过氧化物酶体增殖物激活受体-γ 激动剂，可诱导离体猪视网膜小动脉扩张：一氧化氮和钾通道的作用。
影响因子：
4.4
作者：
Tsuneaki Omae;T. Nagaoka;Ichiro Tanano;A. Yoshida
通讯作者：
A. Yoshida