Flavonols improve thermotolerance in tomato pollen during germination and tube elongation by maintaining ROS homeostasis.
Flavonols improve thermotolerance in tomato pollen during germination and tube elongation by maintaining ROS homeostasis.
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黄酮醇通过维持活性氧稳态来提高番茄花粉在发芽和管伸长过程中的耐热性。
DOI：
10.1101/2023.12.23.573189
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Muday,GloriaK
中科院分区：
文献类型：
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作者：
Postiglione,AnthonyE;Delange,AllisonM;Ali,MohammadFoteh;Houben,Maarten;Wang,EricY;Hahn,StacyL;Roark,ColleenM;Davis,Molly;Reid,RobertW;Pease,JamesB;Loraine,AnnE;Muday,GloriaK
温度升高会损害花粉的性能和繁殖成功率，导致作物产量降低。番茄花色素苷减少突变体具有黄烷酮3羟化酶（F3 H）基因突变，导致黄酮醇抗氧化剂的合成受损。相对于VF 36亲本系，突变体具有降低的花粉性能和结实，这在升高的温度下加重。用野生型F3 H基因或黄酮醇类化合物进行转化，可防止花粉中温度依赖性ROS的积累，并将降低的活力、萌发和管伸长逆转至VF 36水平。用F3 H过表达构建体转化的VF 36防止温度驱动的ROS增加和受损的花粉性能，揭示了黄酮醇合成升高的耐热性结果。虽然柱头有减少的黄酮醇和升高的活性氧，花粉管的生长同样受损，在这两个是和VF 36雌蕊。在最佳温度和胁迫温度下，在整个花粉管伸长的多个时间点，在are、VF 36和VF 36 F3 H过表达系中进行RNA-Seq。在所有基因型中，差异表达基因的数量随着温度升高而增加，其中以基因型中差异表达基因数量最多。这些研究结果表明，潜在的农业干预措施，以打击热诱导的活性氧在花粉中，导致生殖失败的负面影响。一句话总结黄酮醇抗氧化剂通过降低热诱导的活性氧水平和调节花粉转录组中热诱导的变化来减少高温对花粉性能的负面影响。
Elevated temperatures impair pollen performance and reproductive success, resulting in lower crop yields. The Solanum lycopersicum anthocyanin reduced ( are ) mutant has a FLAVANONE 3 HYDROXYLASE ( F3H ) gene mutation resulting in impaired synthesis of flavonol antioxidants. The are mutant has reduced pollen performance and seed set relative to the VF36 parental line, which is accentuated at elevated temperatures. Transformation of are with the wild-type F3H gene, or chemical complementation with flavonols, prevented temperature-dependent ROS accumulation in pollen and reversed are’s reduced viability, germination, and tube elongation to VF36 levels. VF36 transformed with an F3H overexpression construct prevented temperature driven ROS increases and impaired pollen performance, revealing thermotolerance results from elevated flavonol synthesis. Although stigmas of are had reduced flavonols and elevated ROS, the growth of are pollen tubes were similarly impaired in both are and VF36 pistils. RNA-Seq was performed at optimal and stress temperatures in are , VF36, and the VF36 F3H overexpression line at multiple timepoints across pollen tube elongation. Differentially expressed gene numbers increased with duration of elevated temperature in all genotypes, with the largest number in are . These findings suggest potential agricultural interventions to combat the negative effects of heat-induced ROS in pollen that leads to reproductive failure. One sentence summary Flavonol antioxidants reduce the negative impacts of elevated temperatures on pollen performance by reducing levels of heat induced reactive oxygen species and modulation of heat-induced changes in the pollen transcriptome.