The SPFH domain: implicated in regulating targeted protein turnover in stomatins and other membrane-associated proteins
The SPFH domain: implicated in regulating targeted protein turnover in stomatins and other membrane-associated proteins
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DOI：
10.1016/s0968-0004(99)01467-x
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发表时间：
1999-11-01
影响因子：
13.8
通讯作者：
Kyrpides, NC
中科院分区：
文献类型：
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作者：
Tavernarakis, N;Driscoll, M;Kyrpides, NC
图1使用ClustalW程序18生成并使用Boxshade可视化的多序列比对（ISREC，http//www.国际空间研究中心。isb-sib。ch，8080/software/BOX_form。html）。方框标记结构域中最保守的区块，如HMM搜索所识别的19。当序列保守性大于50%时，相同的残基位置以深蓝色突出显示，保守性取代以浅蓝色突出显示。红色箭头表示破坏秀丽隐杆线虫中MEC-2或MEC-1功能的突变。绿色箭头表示已显示对脊椎动物气孔蛋白寡聚化重要的区域。氨基酸位置在比对的边缘中指示。比对结束时括号中的数字表示相应蛋白质的总长度。还显示了源生物。蛋白质名称（和登录号，当不同时）为：PHB_MOUSE，褐家鼠抑制素; PHB-TRYBR，布氏锥虫抑制素; HFLK_ECOLI，大肠杆菌hflK蛋白; HFLK_HAEIN，流感嗜血杆菌hflK蛋白; HFLK_VIBPA，副溶血性弧菌hflK蛋白; MEC2_CAEEL，秀丽隐杆线虫MEC-2蛋白; UNC 1-CAEEL，C.秀丽隐杆线虫ostatin-1蛋白（YMM5_CAEEL）; STOM-人，智人口蛋白（gi| 181184）; AF 1420，闪烁古生球菌膜蛋白（gi| 2649154）; PH 1511，Horikoshii热球菌假设的红细胞带7整合膜蛋白（g3257936）; MTH 1780，热自养甲烷杆菌气孔蛋白样蛋白（gi| 2622911）; UNC24-CAEEL，C.秀丽隐杆线虫α-24蛋白|1353669）; HFLC-THEMA，海栖热袍菌hflC蛋白（gi| 4982405）; HFLCRICPR，普氏立克次氏体hflC蛋白（g3860691）; FLO 1-HUMAN，H.智人flotillin-1（gi| 3599573）; FLO 1-DROME，黑腹果蝇flotillin-1（gi| 3115385）; FLO2-HUMAN，H.智人flotillin-2（gi| 627431）; FLO2-DROME，D.黑腹菌flotillin-2（gi| 3115387）。多序列比对（比对号ds 39774）已保藏于欧洲生物信息学研究所（ftp：//ftp. ebi AC. uk/pub/databases/embl/align/ds39774。dat）。
Figure 1 Multiple sequence alignment generated with the ClustalW program18 and visualized with Boxshade (ISREC, http//www. isrec. isb-sib. ch, 8080/software/BOX_form. html). Boxes mark the most conserved blocks of the domain, as identified with HMM searches19. When sequence conservation is greater than 50%, identical residue positions are highlighted in dark blue and conservative substitutions in light blue. Red arrowheads denote mutations that disrupt the function of MEC-2 or UNC-1 in Caenorhabditis elegans. The green arrowbar denotes the region that has been shown to be important for oligomerization of vertebrate stomatin. Amino acid positions are indicated in the margins of the alignment. Numbers in parentheses at the end of the alignment denote the total length of the corresponding protein. Source organisms are also shown. Protein names (and accession numbers, when different) are: PHB_MOUSE, Rattus norvegicus prohibitin; PHB-TRYBR, Trypanosoma brucei prohibitin; HFLK_ECOLI, Escherichia coli hflK protein; HFLK_HAEIN, Haemophilus influenzae hflK protein; HFLK_VIBPA, Vibrio parahaemolyticus hflK protein; MEC2_CAEEL, Caenorhabditis elegans MEC-2 protein; UNC1-CAEEL, C. elegans UNC-1 protein (YMM5_CAEEL); STOM-HUMAN, Homo sapiens stomatin (gi| 181184); AF1420, Archaeoglobus fulgidus membrane protein (gi| 2649154); PH1511, Pyrococcus horikoshii hypothetical erythrocyte band 7 integral membrane protein (g3257936); MTH1780, Methanobacterium thermoautotrophicum stomatin-like protein (gi| 2622911); UNC24-CAEEL, C. elegans UNC-24 protein (gi| 1353669); HFLC-THEMA, Thermotoga maritima hflC protein (gi| 4982405); HFLCRICPR, Rickettsia prowazekii hflC protein (g3860691); FLO1-HUMAN, H. sapiens flotillin-1 (gi| 3599573); FLO1-DROME, Drosophila melanogaster flotillin-1 (gi| 3115385); FLO2-HUMAN, H. sapiens flotillin-2 (gi| 627431); FLO2-DROME, D. melanogaster flotillin-2 (gi| 3115387). A multiple sequence alignment (alignment number ds39774) has been deposited with the European Bioinformatics Institute (ftp://ftp. ebi. ac. uk/pub/databases/embl/align/ds39774. dat).