microRNAs facilitate comprehensive responses of Bathymodiolinae mussel against symbiotic and nonsymbiotic bacteria stimulation
microRNAs facilitate comprehensive responses of Bathymodiolinae mussel against symbiotic and nonsymbiotic bacteria stimulation
复制标题
microRNA促进Bathymodiolinae贻贝对共生和非共生细菌刺激的综合反应
DOI：
10.1016/j.fsi.2021.10.025
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Chaolun Li
中科院分区：
文献类型：
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作者：
Hao Chen;Minxiao Wang;Huan Zhang;Hao Wang;Li Zhou;Zhaoshan Zhong;Lei Cao;Chao Lian;Yan Sun;Chaolun Li
Bathymodiolinae 贻贝是冷泉和热液喷口的优势物种，可能在鳃细菌细胞中含有内共生菌。然而，多年来，共生背后的机制基本上仍未被披露。在本研究中，使用富集的共生甲烷氧化细菌 MOB 或非共生弧菌对细菌挑战期间的免疫相关基因和 miRNA 的整体表达模式进行了调查。结果，发现多种模式识别受体在细菌攻击后 12 小时和 24 小时有差异表达，并且在刺激之间明显聚集。在 MOB 或弧菌挑战期间，数十个免疫效应器和信号传感器也被同时调节。在鳃中总共鉴定出 459 个 miRNA，其中一些在 MOB 或非共生细菌攻击后差异表达。多种免疫相关基因被注释为上述差异表达miRNA的靶基因。因此，在共生或非共生细菌攻击中，包括免疫识别、溶酶体活性和细菌吞噬在内的生物过程被认为由 miRNA 动态调节。有人建议G.贻贝可以对入侵的病原体保持强大的免疫反应，同时与免疫相关基因和 miRNA 的化学合成细菌建立共生关系。
Bathymodiolinae mussels are dominant species in cold seeps and hydrothermal vents and could harbor endosymbionts in gill bacteriocytes. However, mechanisms underlying the symbiosis have remained largely undisclosed for years. In the present study, the global expression pattern of immune-related genes and miRNAs were surveyed inGigantidas platifronsduring bacterial challenges using enriched symbiotic methane oxidation bacteria MOBs or nonsymbioticVibrio. As a result, multiple pattern recognition receptors were found differentially expressed at 12 h and 24 h post bacteria challenges and distinctly clustered between stimulations. Dozens of immune effectors along with signal transducers were also modulated simultaneously during MOB orVibriochallenge. A total of 459 miRNAs were identified in the gill while some were differentially expressed post MOB or nonsymbiotic bacteria challenge. A variety of immune-related genes were annotated as target genes of aforesaid differentially expressed miRNAs. As a result, biological processes including the immune recognition, lysosome activity and bacteria engulfment were suggested to be dynamically modulated by miRNAs in either symbiotic or nonsymbiotic bacteria challenge. It was suggested thatG. platifronsmussels could maintain a robust immune response against invading pathogens while establishing symbiosis with chemosynthetic bacteria with the orchestra of immune-related genes and miRNAs.