High prevalence of 1762T 1764A mutations in the basic core promoter of hepatitis B virus isolated from black Africans with hepatocellular carcinoma compared with asymptomatic carriers
High prevalence of 1762T 1764A mutations in the basic core promoter of hepatitis B virus isolated from black Africans with hepatocellular carcinoma compared with asymptomatic carriers
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DOI：
10.1002/hep.510290336
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发表时间：
1999-03-01
期刊：
影响因子：
13.5
通讯作者：
Kew, MC
中科院分区：
文献类型：
--
作者：
Baptista, M;Kramvis, A;Kew, MC
这项研究的目的是确定基本核心启动子和增强子II区域的突变，这些突变可能导致HBVe抗原(HBeAg)阴性的表型，并有助于该病毒的非洲黑人携带者发生肝癌。基本核心启动子/增强子II与X基因重叠。对47例无症状携带者和50例肝细胞癌患者的血清、28例肿瘤组织和10例非肿瘤肝组织的HBVDNA进行了直接扩增和测序。增强子II区不与基本核心启动子重叠的部分在所有样本中都是完全保守的。在80%的序列中发现了碱性核心启动子1809和1812核苷酸的错义突变，可能代表了南部非洲分离株的野生型序列。肝细胞癌患者基本核心启动子的核苷酸和氨基酸差异均高于无症状携带者(P
The purpose of this study was to identify mutations in the basic core promoter and enhancer II region of the hepatitis B virus (HBV) that might cause the HBV e antigen (HBeAg)-negative phenotype and contribute to hepatocarcinogenesis in black African carriers of the virus. The basic core promoter/enhancer II overlaps with the X gene. HBV DNA from serum of 47 asymptomatic carriers and 50 patients with hepatocellular carcinoma and from 28 tumor and 10 nontumor liver tissues was amplified and sequenced directly. That part of the enhancer II region not overlapping the basic core promoter was completely conserved in all samples. Missense mutations at nucleotides 1809 and 1812 in the basic core promoter were found in 80% of all sequences and may represent wild-type sequence in Southern African isolates. Nucleotide and amino acid divergences were higher in the basic core promoter of hepatocellular carcinoma patients when compared with asymptomatic carriers (P