Structural, Mechanical, and Dynamical Variability of the Actin Cortex in Living Cells
Structural, Mechanical, and Dynamical Variability of the Actin Cortex in Living Cells
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DOI：
10.1016/j.bpj.2015.01.016
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发表时间：
2015-03-24
影响因子：
3.4
通讯作者：
Scheuring, Simon
中科院分区：
文献类型：
--
作者：
Eghiaian, Frederic;Rigato, Annafrancesca;Scheuring, Simon
在真核细胞中，一个基于肌动蛋白的皮层排列在质膜的内小叶上，赋予细胞重要的机械和功能特性。不幸的是，它还没有能够在活细胞中的高横向分辨率研究肌动蛋白皮质的结构动力学。在这里，我们进行了原子力显微镜延时成像和机械映射的肌动蛋白在活细胞的皮质在高横向和时间分辨率。皮质肌动蛋白丝采用了可辨别的安排，从大的平行束与低连接到一个紧密的网络的短丝。这些架构的混合导致了具有特定连接性、机械响应和动态行为显著差异的协调皮层网络。
In eukaryotic cells, an actin-based cortex lines the inner leaflet of the plasma membrane, endowing the cells with crucial mechanical and functional properties. Unfortunately, it has not been possible to study the structural dynamics of the actin cortex at high lateral resolution in living cells. Here, we performed atomic force microscopy time-lapse imaging and mechanical mapping of actin in the cortex of living cells at high lateral and temporal resolution. Cortical actin filaments adopted discernible arrangements, ranging from large parallel bundles with low connectivity to a tight meshwork of short filaments. Mixing of these architectures resulted in attuned cortex networks with specific connectivity, mechanical responses, and marked differences in their dynamic behavior.